Comprehensive Assignments of Extraction, Isolation and Characterization of Taraxerol from Bark Annona reticulata L. and Chemopreventive Effect on Human Prostate Cancer Cell Lines (lncap and pc-3)

Abstract

Prostate Cancer (PC) ruins a foremost cause of death of males in the US as well its growth rate is increased in the rest of the world. The current learning aims to perform a preliminary photochemical analysis by the successive extraction of the bark of Annona reticulata L. using petroleum ether, chloroform, and ethanol. The isolation, structure elucidation and identification of Taraxerol and check up in vitro study in prostate carcinoma. The structure was elucidated by spectroscopic techniques included Thin Layer chromatography (TLC) and High Performance Liquid Chromatography (HPLC), UV and Gas Chromatography-Mass Spectrometric (GC-MS). The prostate cell lines, LNCaP and PC-3 cell lines was cultured and antiproliferative effect by MTT Method, Neutral red cytotoxicity, measurement of LDH release, determinations of apoptosis by Acridine Orange (AO) and Ethidium Bromide (EB) double staining. Inhibition of protein denaturation, caspase levels by indirect ELISA and DNA fragmentation was performed. Investigation of the phytochemical summary on the bark of A. reticulata L. reports the occurrence of flavonoids, saponins, triterpenoid and tannins. In-vitro experiments show the selected compound exhibited of cytotoxicity against the cancer cell lines. An increase in caspase activity or caspase levels is generally considered as indicators of cellular apoptosis. The compounds to prevent heat associated denaturation of albumin are measured as a screening method for assessing anti-inflammatory potential of compounds.