Identification of a laccase gene involved in shell periostracal tanning of the clam Meretrix petechialis

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2019-06-06 DOI:10.3354/AB00709

Xin Yue, Zhang Shujing, Jiajia Yu, Liu Baozhong

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引用次数: 4

Abstract

Tanning is a complex extracellular process that is a mechanism for stabilizing proteinaceous extracellular structures. Phenoloxidases play important roles in cross-linking during tanning, and laccase (EC 1.10.3.2) is a member of the phenoloxidase enzyme class. In this study, we identified a laccase gene (MpLac) from the clam Meretrix petechialis and found that MpLac might be involved in shell periostracal tanning of clams. Using whole-mount in situ hybridization, we found that MpLac mRNA in the larval clam was mainly expressed in the mantle edge. In the adult clam, our quantitative real-time PCR results showed that the mantle was also a tissue with a high MpLac expression level; in addition, by combining the results of fluorescence in situ hybridization, H&E staining and transmission electron microscopy, we found that the inner epithelium of the outer fold of the mantle edge, which is involved in periostracum formation, was the exact region in which MpLac mRNA was expressed. Furthermore, knocking down the expression of MpLac by RNA interference (RNAi) bleached the new shell periostracum. All of our results suggest the involvement of MpLac in shell periostracal tanning of M. petechialis.

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蛤壳周鞣制过程中漆酶基因的鉴定

鞣制是一个复杂的细胞外过程，是一种稳定蛋白质细胞外结构的机制。酚氧化酶在鞣制过程中起着重要的交联作用，漆酶(EC 1.10.3.2)是酚氧化酶类的一员。在本研究中，我们从蛤蚌中鉴定出一个漆酶基因(MpLac)，并发现MpLac可能参与了蛤壳膜鞣制过程。利用全贴装原位杂交技术，我们发现mplacmrna在蛤蚌幼体中主要在地幔边缘表达。我们的实时荧光定量PCR结果显示，在成年蛤中，地幔也是mplace高表达的组织;此外，结合荧光原位杂交、H&E染色和透射电镜结果，我们发现，参与骨膜形成的地幔边缘外褶皱内上皮正是mplacmrna表达的区域。此外，通过RNA干扰(RNAi)抑制mplaca的表达，使新壳膜脱色。我们所有的结果都表明MpLac参与了斑点分枝杆菌的壳周鞣制。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.