Histomorphometric analysis of pulp submitted to the action of bromelain associated with irrigation solutions

IF 0.5 Q4 DENTISTRY, ORAL SURGERY & MEDICINE

Giornale Italiano di Endodonzia Pub Date : 2019-06-11 DOI:10.32067/GIE.2019.33.01.03

V. Caetano, E. Alves, L. C. França, D. Lenardo, Victor-Brito-Dantas Martins, J. Galeno, Jefferson S. Oliveira, D. Vasconcelos

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引用次数: 1

Abstract

Aim: The objective of this study was to evaluate the tissue disgregation capacity of pulp in different solutions associated or not with bromelain. Methodology: Fifty bovine mandibular incisors were used, divided into five groups: C: Control, H: sodium hypochlorite 2.5%, B: bromelain, BL: bromelain + L-cysteine, BH: bromelain + sodium hypochlorite 2.5%. The pulp fragments were stored at -20 °C, washed with saline solution and moved to 25°C in eppendorf tubes with 1 ml of each substance. For the histomorphometric evaluation, the pulp fragments were prepared as histological routine, observed with optical microscopy and evaluated using ImageJ 1.49 software. Results: The histomorphometric results showed that, in C: there was no alteration per tissue; H: there was a peripheral and central tissue disintegration; B: there was a protein and cellular degradation pattern, from the peripheral to the central area; BL: there was a central degradation. In BH, showed an effect enhanced by bromelain, when related to sodium hypochlorite, showing greater tissue degradation. Conclusions: The association of bromelain with sodium hypochlorite potentiated the disintegration action of the pulp, opening possibilities for several clinical applications.

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灌溉水中菠萝蛋白酶作用下的果肉组织形态学分析

目的:本研究的目的是评价不同溶液中与菠萝蛋白酶相关或不相关的牙髓组织分散能力。方法:选用50只牛下颌骨切牙，分为5组:C:对照组，H:次氯酸钠2.5%组，B:菠萝蛋白酶，BL:菠萝蛋白酶+ l -半胱氨酸，BH:菠萝蛋白酶+次氯酸钠2.5%组。牙髓碎片在-20°C保存，用生理盐水洗涤，并在辅助管中移至25°C，每种物质各1 ml。组织形态学评价采用牙髓碎片作为组织学常规，光学显微镜观察，ImageJ 1.49软件评价。结果:组织形态学结果显示，C组各组织无明显改变;H:有外周和中央组织解体;B:有蛋白和细胞降解模式，从周围到中心区域;BL:有一个核心的退化。在BH中，菠萝蛋白酶的作用增强，当与次氯酸钠相关时，表现出更大的组织降解。结论:菠萝蛋白酶与次氯酸钠的结合增强了牙髓的崩解作用，为多种临床应用开辟了可能性。

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来源期刊

Giornale Italiano di Endodonzia DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

0.90

自引率

25.00%

发文量

审稿时长

24 weeks

期刊介绍： The Giornale Italiano di Endodonzia was founded in 1987 and is the official journal of the Italian Society of Endodontics (SIE). It is a peer-reviewed journal publishing original articles on clinical research and/or clinical methodology, case reports related to Endodontics. The Journal evaluates also contributes in restorative dentistry, dental traumatology, experimental pathophysiology, pharmacology and microbiology dealing with Endodontics.