Effect of Oleanolic and Ursolic Acids Isolated from Loquat (Eriobotrya) on the Growth of Human Lymphoid Leukemia Cells

Food Science and Technology International, Tokyo Pub Date : 1998-11-25 DOI:10.3136/FSTI9596T9798.4.282

T. Komiya, Y. Achiwa, H. Katsuzaki, K. Imai, S. Sakurai, K. Urakawa, Kazuko Ohnishi, T. Adachi, Tetsuya Yamada, H. Hibasami

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引用次数: 10

Abstract

We have identified two triterpene carboxylic acids of oleanolic acid and ursolic acid isolated from the leaves of loquat (Eriobotrya) by NMR spectroscopy. We have investigated the effects of oleanolic acid and ursolic acid on the growth of lymphoid leukemia Molt 4B cells and obtained results in which both compounds inhibited the proliferation of Molt 4B cells. In an in vitro experiment, ursolic acid inhibited ornithine decarboxylase (ODC) activity by 25.9% and 39.7% at the concentrations of 1 mM and 2 mM, respectively, while oleanolic acid inhibited ODC activity to a lesser extent than did ursolic acid. Both compounds slightly suppressed S-adenosylmethionine decarboxylase (AdoMetDC) activity. Polyamine contents were decreased in these compound-treated cells. These findings demonstrate that growth suppression of human Iymphoid Molt 4B cells by ursolic acid and oleanolic acid may result from the depletion of polyamines caused by the inhibition of ODC and AdoMetDC activities.

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枇杷齐墩果酸和熊果酸对人淋巴白血病细胞生长的影响

用核磁共振光谱法鉴定了枇杷叶中齐墩果酸和熊果酸两种三萜羧酸。我们研究了齐墩果酸和熊果酸对淋巴细胞白血病Molt 4B细胞生长的影响，结果发现这两种化合物都抑制了Molt 4B细胞的增殖。在体外实验中，熊果酸在1 mM和2 mM浓度下对鸟氨酸脱羧酶(ODC)活性的抑制作用分别为25.9%和39.7%，齐墩果酸对ODC活性的抑制作用小于熊果酸。两种化合物均能轻微抑制s -腺苷甲硫氨酸脱羧酶(AdoMetDC)活性。这些化合物处理的细胞中多胺含量降低。这些发现表明熊果酸和齐墩果酸对人淋巴细胞4B生长的抑制可能是由于ODC和AdoMetDC活性的抑制导致多胺的消耗。

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Food Science and Technology International, Tokyo

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