A portable FTIR-ATR process analyzer - online fermentation control

Proceedings of IEEE Sensors, 2004. Pub Date : 2004-01-01 DOI:10.1109/ICSENS.2004.1426274

J. Schnoller, B. Lendl

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引用次数: 7

Abstract

A mid-IR FT spectrometer equipped with a horizontal attenuated total reflection (ATR) unit has been used for fermentation monitoring. The ATR element has been incorporated in a flow cell connected to the fermentation process under investigation via a flexible computer controlled flow system for online monitoring. Measurement of dissolved components is achieved while pumping the sampled fermentation broth through the flow cell. Upon stopping the flow, the microorganisms can be monitored subsequently as they settle on top of the horizontal ATR element. Because of the surface sensitivity of ATR spectroscopy, the cleanness of the ATR surface must be guaranteed throughout a prolonged operating time. For certain bioprocesses, the formation of bio-films on the ATR surface was identified as an important difficulty as they cannot be removed by simple rinsing with distilled water or simple washing solutions such as NaHCO/sub 3/. Therefore, different chemical solutions have been tested for efficient bio-film removal. Among the studied solutions, the use of oxidizing agents (e.g. H/sub 2/O/sub 2/) in concentrations around 2% proved most effective.

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便携式FTIR-ATR过程分析仪-在线发酵控制

采用中红外FT光谱仪，配备水平衰减全反射(ATR)装置，对发酵过程进行了监测。ATR元件已通过灵活的计算机控制流动系统集成到与发酵过程相连接的流动池中，用于在线监测。溶解成分的测量是在泵送取样的发酵液通过流动池时实现的。在停止流动后，微生物可以随后被监测，因为它们沉降在水平ATR元件的顶部。由于ATR光谱的表面敏感性，必须保证ATR表面的清洁度。对于某些生物工艺，在ATR表面形成生物膜被认为是一个重要的困难，因为它们不能通过简单的蒸馏水或简单的洗涤溶液(如NaHCO/ sub3 /)来去除。因此，不同的化学溶液已被测试为有效的生物膜去除。在所研究的溶液中，使用浓度在2%左右的氧化剂(例如H/sub - 2/O/sub - 2/)被证明是最有效的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Proceedings of IEEE Sensors, 2004.

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