Cytogenetic and immunological efficacy of nicotiflorin and rutin combination on gamma irradiated rats

IF 0.4 4区医学 Q4 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

International Journal of Radiation Research Pub Date : 2022-04-01 DOI:10.52547/ijrr.20.2.29

M. R. Mohammed, M. M. Ahmed, S. A. Montaser

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引用次数: 1

Abstract

Background : Cytogenetic and immunological damages after ionizing radiation exposure are critical factors that lead to many different events and consequences cascade reactions starting from inflammation, ending with cell damage. These biological events if not well controlled will lead to deleterious effects and cancer. The present study aimed to evaluate the efficacy of the Nicotiflorin and Rutin (NR) combination against drastic effects resulted from γ-irradiation. Materials and Methods : Rats were divided into four groups. (Control) group, (NR) group: treated with 20mg/kg body weight orally daily one dose for two weeks, (Irrad) group: rats exposed to 6Gy as a single dose, and (NR+ Irrad) group which treated with NR combination before irradiation. Detection of DNA damage was done with the Micronucleus test (MN) and Comet assay. Immunological responses were detected by assessing inflammatory cytokines Interluekines-1β (IL-1β), tumor necrosis factor (TNF-α), and homeostasis maintenance cytokines (IL-6 & IL-10). Results : Irradiated group recorded a significant increase in micronuclei (MNi) incidences and significant DNA fragmentation. As well, immunological parameters displayed a significant increase in all measured interleukins except IL-10 which recorded a significant decline. The group injected with NR before irradiation showed significant improvement in all measured parameters. Conclusion : The efficacy of the NR combination may be attributed to its dual protective effects (cytogenetic and immunological enhancement) against damage caused by γ-irradiation.

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烟碱与芦丁联合对γ辐照大鼠细胞遗传学和免疫学的影响

背景:电离辐射暴露后的细胞遗传学和免疫学损伤是导致许多不同事件和后果的关键因素，从炎症开始，以细胞损伤结束。这些生物事件如果不能很好地控制，将导致有害的影响和癌症。本研究旨在评价烟florin和芦丁(NR)联合使用对γ辐照引起的剧烈反应的疗效。材料与方法:将大鼠分为四组。(对照)组、(NR)组:按20mg/kg体重每日口服1次，连续2周;(Irrad)组:按6Gy单次给药;(NR+ Irrad)组辐照前用NR联合给药。用微核试验(MN)和彗星试验检测DNA损伤。免疫反应通过评估炎症因子介素-1β (IL-1β)、肿瘤坏死因子(TNF-α)和稳态维持因子(IL-6和IL-10)来检测。结果:辐照组小鼠微核(MNi)发生率显著升高，DNA断裂明显。此外，除IL-10显著下降外，免疫参数显示所有测量的白细胞介素均显著增加。辐照前注射NR组各项指标均有显著改善。结论:NR联合用药对γ辐照损伤具有双重保护作用(细胞遗传学和免疫增强)。

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来源期刊

International Journal of Radiation Research RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-

CiteScore

1.10

自引率

33.30%

发文量

期刊介绍： International Journal of Radiation Research (IJRR) publishes original scientific research and clinical investigations related to radiation oncology, radiation biology, and Medical and health physics. The clinical studies submitted for publication include experimental studies of combined modality treatment, especially chemoradiotherapy approaches, and relevant innovations in hyperthermia, brachytherapy, high LET irradiation, nuclear medicine, dosimetry, tumor imaging, radiation treatment planning, radiosensitizers, and radioprotectors. All manuscripts must pass stringent peer-review and only papers that are rated of high scientific quality are accepted.