Effect of soya-milk based extender on the physico-morphological parameters of Murrah bull semen during cryopreservation

S. Hussain, Sudershan Kumar, M. Iqbal, T. Ahmed
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引用次数: 1

Abstract

Present investigation was undertaken on three buffalo bulls (Murrah) to study the effect of cryopreservation using Soya-milk extender. A total of 18 collections from three bulls (six collections per bull) were utilized for the study. After initial evaluation, each semen sample was diluted in two different extenders viz. Tris egg yolk extender which acted as control and Soya-milk extender as experimental. Various sperm parameters were studied at equilibration and post-thaw stages. There was no significant difference for percent head abnormalities, percent midpiece abnormalities and acrosome integrity of spermatozoa among extenders at different stages of freezing. The progressive motility and live sperm percentage was significantly higher for Tris egg yolk extender at dilution, equilibration and postthaw stages, but the values for Soya-Milk were also within acceptable limits at all stages of freezing as compared to Tris egg yolk extender. Furthermore the percent tail abnormalities and total sperm abnormalities were significantly higher for soya-milk extender at post thaw. In conclusion the experimental extender could be used as an alternate plant based extender in the cryopreservation of bubaline semen.
豆浆膨化剂对默拉公牛精液低温保存过程中物理形态参数的影响
本研究以3头水牛为试验对象,研究豆浆膨化剂对其冷冻保存的影响。本研究共使用了来自3头公牛的18个标本(每头公牛6个标本)。初步评价后,每个精液样品用两种不同的扩展剂稀释,即Tris蛋黄扩展剂作为对照,豆浆扩展剂作为实验。在平衡和解冻后阶段研究了精子的各种参数。不同冷冻阶段延长者的精子头部畸形率、中段畸形率和顶体完整性无显著差异。在稀释、平衡和解冻后阶段,Tris卵黄扩展剂的渐进式活力和活精子率显著高于Tris卵黄扩展剂,但与Tris卵黄扩展剂相比,豆浆在所有冷冻阶段的数值也在可接受的范围内。豆浆膨化剂解冻后的尾畸形率和总精子畸形率显著高于其他膨化剂。实验结果表明,该延长剂可作为植物性延长剂用于丁胆碱精液的低温保存。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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