Genetic Improvement of Drought Tolerance in North Eastern Chakhao Amubi rice Through Marker Assisted Selection

Madras Agricultural Journal Pub Date : 2022-01-01 DOI:10.29321/maj.10.000583

R. Kambale

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Abstract

Drought ranks the top among the abiotic stresses affecting rice production and sustaining rice production warrants the development of drought tolerant rice cultivars. This study was aimed at developing recombinant inbred lines of Chakhao Amubi possessing drought tolerant QTLs of Apo. A RIL population developed between Chakhao amubi (popular rice in Manipur) and Apo (a drought tolerant cultivar) was genotyped using SSR markers linked to major drought tolerant QTLs, the positive lines were screened for grain characteristics and aroma. Foreground analysis of F2 (117 progenies) population using SSR markers RM11885 (qDTY1.1), RM7426 (qDTY2.1), RM16030 (qDTY3.1) and BADEX7-5 (OsBADH2 loci controlling aroma) resulted in the identification of 25 progenies harboring all the three drought tolerant QTLs (qDTY1.1, qDTY2.1 and qDTY3.1) and aroma, 11 progenies were found to harbour two target QTLs and aroma and two progenies were found to harbour any one of the drought QTL and aroma. Agronomic evaluation of RILs (F4) identified 11 superior RILs possessing reduced duration, high yield, and all four QTLs for further evaluation and breeding applications.

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利用标记辅助选择对东北茶好稻抗旱性的遗传改良

干旱是影响水稻生产的主要非生物胁迫，培育耐旱水稻品种是水稻持续生产的必然要求。本研究旨在培育具有Apo耐旱qtl的茶蒿自交系。利用主要耐旱qtl连锁的SSR标记，对Chakhao amubi(曼尼普尔地区常见水稻)和Apo(耐旱品种)亲本RIL群体进行了分型，并对阳性株系进行了籽粒性状和香气筛选。利用SSR标记RM11885 (qDTY1.1)、RM7426 (qDTY2.1)、RM16030 (qDTY3.1)和BADEX7-5 (OsBADH2控制香气位点)对F2(117个后代)群体进行前景分析，鉴定出25个后代同时拥有3个耐旱QTL (qDTY1.1、qDTY2.1和qDTY3.1)和香气，其中11个后代同时拥有两个目标QTL和香气，2个后代同时拥有干旱QTL和香气中的任何一个。rls农艺评价(F4)鉴定出11个持续时间短、产量高的优良rls和4个qtl，可供进一步评价和育种应用。

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Madras Agricultural Journal

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