X-ray microtomography of collagen and polylactide scaffolds in liquids.

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference Pub Date : 2015-01-01 DOI:10.1109/EMBC.2015.7319668

M. Hannula, A. Haaparanta, Ilmari Tamminen, A. Aula, M. Kellomaki, J. Hyttinen

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引用次数: 1

Abstract

Methods to image and assess the microstructure of polymer based biomaterials in liquid phase, for example cell culture medium, are well warranted. X-ray microtomography could provide a mean to visualize and analyze such structures. However, the density of such polymers is close to that of water and hence the X-ray contrast is poor. Moreover, if the biomaterials contain cells and are dried, the cell morphology may be distorted. In this paper we test phosphotungstic acid (PTA) staining to improve the contrast. We imaged collagen and PLA samples with μCT in air, water and alcohol. The methods were compared visually and with contrast to noise ratio calculated from the images. Our results demonstrate that with alcohol the PLA can be imaged also in liquid phase. PTA staining seems to be a good method to increase the contrast for collagen in μCT imaging.

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液体中胶原蛋白和聚乳酸支架的x射线显微断层扫描。

在液相中成像和评估聚合物基生物材料微观结构的方法，例如细胞培养基，是很有必要的。x射线显微断层扫描可以提供一种可视化和分析这些结构的方法。然而，这种聚合物的密度接近于水的密度，因此x射线对比很差。此外，如果生物材料含有细胞并将其干燥，则细胞形态可能会扭曲。本文对磷钨酸(PTA)染色进行了试验，以提高对比度。我们用μCT在空气、水和酒精中对胶原蛋白和聚乳酸进行成像。并与图像计算的噪声比进行了对比。我们的结果表明，在酒精的作用下，PLA也可以在液相中成像。在μCT成像中，PTA染色似乎是一种很好的增加胶原对比的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference

CiteScore

2.20

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0.00%

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