Sumbawa Horse Milk as a Prevention of Inflammatory Bowel Disease (IBD) in Animal Model based on IL-6 Expression and Gastric Histopathology

Abstract

: Sumbawa horse milk contains bioactive components as exogenous antioxidants, anti-inflammatory andantimicrobial which can prevent Inflammatory Bowel Disease. IBD is characterized by ongoing inflammation of the digestive tract that could changed gastric histology and trigger pro-inflammatory cytokines. The purpose of this study was to observe the preventive effects of Sumbawa horse milk on gastric organs inflammation in IBD animal models. Twenty male rats, 8 weeks, 150-200 grams BW, divided into five treatment groups; K- group (negative control), indometacin induced group; i.e K+ (positive group without horse milk), P1(horse milk dose 0.5 mL/rat), P2 (horse milk dose1.0 mL/rat), and P3(horse milk dose 1.5mL/rat). Flowcytometry method was conducted to observe IL-6 expression, and then analyzed with one way ANOVA followed by a tukey test (α 95%). While the gastric histopathological was analyzed descriptively. The results showed that IL-6 expression were significantly lower (p <0.05) in P2 dan P3 compared with K+group. Whereas in the histological result, the p3 group was able to prevent histopathological damage of the gastric organs compared to other treatment groups. The conclusion of the study is Sumbawa horse milk able to prevent increased of IL-6 and gastric mucosal cell erosion, with the best dose is 1.5 mL/rat.