Identification of Vietnamese native Dendrobium species based on ribosomal DNA internal transcribed spacer sequence

Abstract

This study aimed to identify plant sources of Vietnamese Dendrobium from different regions based on sequences of the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA. We constructed an ITS1-5.8S-ITS2 sequence database of 32 Vietnamese native Dendrobium species. Comparison between sequencings of 32 Vietnamese native Dendrobium and Dendrobium species in the world by using ITS sequences, we have determined exactly the same pattern 23/32 of Dendrobium species. In the remaining 9 Vietnamese native Dendrobium varieties, 4 varieties were re-edited Latin names such as Hoang Thao tram Trang, D15 (Hoang Thao Tam Dao), D24 (Hoang Thao Vay rong la nho), and D25 (Hoang Thao Vay rong la trung); 5 samples have not been pinpointed as the same form including D11 (Hoang Thao Xoan), D12 (Hoang Thao Kieu tim), D14 (Hoang Thao Kieu trang), D19 (Hoang Thao Kieu trang Dong Nai), and D26 (Hoang Thao Thuy tien mo ga) species.