Effect of Heat Shock Treatment on Microspore Embryogenesis in Brassica oleracea Species

Abstract

Heat shock treatments are widely used to induce microspore embryogenesis in Brassica species. In this study, the effect of high temperature treatment (32 °C and 35 °C for 2 days) on microspore embryogenesis was investigated in six genotypes of Turkish white head cabbage (Yalova-1, Ercis, 177 C, 177 T, 531 C, 538 C), three genotypes of Turkish kale (Balkaya, Yanmaz, Karadere 077) and five commercial F1 ornamental kale hybrids (Red Piegon, Victoria Piegon, Red Chidori, white Kamome, and Pink Kamome). Microspore-derived embryos formation differed depending on genotype and high temperature. The highest embryo yield was obtained as 9.92 embryo per petri dish in cv. Yalova-1, 11.13 embryo per petri dish in Pink Kamome F1 at 32 °C, and 5.63 embryo per petri dish in cv. Karadere 077 at 35 °C.