Listeriolysin O, but not Murine E-cadherin, is Involved in Invasion of Listeria monocytogenes into Murine Liver Parenchymal Cells.

Q3 Immunology and Microbiology

Open Microbiology Journal Pub Date : 2015-07-31 eCollection Date: 2015-01-01 DOI:10.2174/1874285801509010081

Yu-Ju Kanayama, Masakazu Kaneko, Yoshiko Emoto, Masashi Emoto

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引用次数: 3

Abstract

Human E-cadherin and listeriolysin O (LLO) are involved in invasion of Listeria monocytogenes into human liver parenchymal cells (LPC). Yet, it remains to be determined whether murine E-cadherin and LLO participate in invasion of L. monocytogenes into murine LPC. In the present study, involvement of murine E-cadherin and LLO in invasion of L. monocytogenes into murine LPC was investigated. Murine E-cadherin was expressed on murine LPC, but the expression became undetectable by insertion of transgene of Simian virus 40 large T antigen. Although invasion of L. monocytogenes into murine LPC was found regardless of murine E-cadherin expression, infection rate of L. monocytogenes being unable to secrete LLO was lower than that of L. monocytogenes being capable of secreting LLO. Our RESULTS verify that invasion of L. monocytogenes into murine LPC occurs independently of murine E-cadherin and indicate that LLO participates in invasion of L. monocytogenes into murine LPC.

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李斯特菌溶素O，而不是小鼠E-钙粘蛋白，参与了单核细胞增多性李斯特菌对小鼠肝实质细胞的侵袭。

人E-钙粘蛋白和李斯特菌溶素O（LLO）参与了单核细胞增多性李斯特菌对人肝实质细胞（LPC）的侵袭。然而，小鼠E-钙粘蛋白和LLO是否参与了李斯特菌对小鼠LPC的侵袭仍有待确定。在本研究中，研究了小鼠E-钙粘蛋白和LLO在李斯特菌入侵小鼠LPC中的作用。小鼠E-钙粘蛋白在小鼠LPC上表达，但通过插入猿猴病毒40大T抗原的转基因而检测不到该表达。尽管无论小鼠E-钙粘蛋白表达如何，都发现了单核细胞增多性李斯特菌对小鼠LPC的侵袭，但不能分泌LLO的单核细胞增生性李斯特菌的感染率低于能够分泌LLO。我们的结果证实，单核细胞增多性李斯特菌对小鼠LPC的入侵独立于小鼠E-钙粘蛋白发生，并表明LLO参与了单核细胞增生性李斯特菌入侵小鼠LPC。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Open Microbiology Journal Immunology and Microbiology-Immunology and Microbiology (all)

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： The Open Microbiology Journal is a peer-reviewed open access journal which publishes research articles, reviews/mini-reviews, case studies, guest edited thematic issues and short communications/letters covering theoretical and practical aspects of Microbial systematics, evolutionary microbiology, immunology, virology, parasitology , bacteriology, mycology, phycology, protozoology, microbial ecology, molecular biology, microbial physiology, biochemistry, microbial pathogenesis, host-microbe interaction, systems microbiology, synthetic microbiology, bioinformatics. The Open Microbiology Journal , a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and freely available to researchers worldwide.