Ashok Patidar , Divanshu Shukla , Neelam Bodhale, Bhaskar Saha
{"title":"TLR2, TLR3, TLR4, TLR9 and TLR11 expression on effector CD4+ T-cell subsets in Leishmania donovani infection","authors":"Ashok Patidar , Divanshu Shukla , Neelam Bodhale, Bhaskar Saha","doi":"10.1016/j.exppara.2023.108645","DOIUrl":null,"url":null,"abstract":"<div><p>T-cells play a central role in cell-mediated immunity. While activation of T-cells is major histocompatibility-restricted, the Toll-like receptors (TLRs)- a family of proteins that recognize conserved molecular patterns present on the pathogens-are not well-studied for their expression and function in T-cells. As any association of TLR expression profiles with an effector T-cell subset is unknown, we analyze BALB/c mice-derived CD4<sup>+</sup> and CD8<sup>+</sup> T-cells’ TLR expression profiles. We report: CD4<sup>+</sup>t-bet<sup>+</sup><span> T-cells are frequent in TLR2</span><sup>Low</sup><span>TLR3</span><sup>High</sup><span>TLR4</span><sup>Low</sup> subpopulation, CD4<sup>+</sup><span>GATA3</span><sup>+</sup> T-cells are frequent within the cells with intermediate expression of TLR2, TLR3, TLR4 and TLR11, CD4<sup>+</sup>FoxP3<sup>+</sup> T-cells in TLR2<sup>High</sup>TLR3<sup>High</sup> cells whereas CD4<sup>+</sup>RORγt <sup>+</sup> T-cells are frequent in TLR2<sup>Low</sup>TLR3<sup>Low</sup>TLR4<sup>Low</sup>TLR11<sup>Low</sup> cells. CD4<sup>+</sup> effector T-cell subsets may therefore show association with TLRs- TLR3, in particular-expression. In <span><em>Leishmania donovani</em></span> infection in BALB/c mice, TLR3 expression on both CD4<sup>+</sup> and CD8<sup>+</sup> T-cells is reduced. Poly-I:C, a TLR3 ligand, do not have any distinctive effects on the CD4<sup>+</sup> effector T-cell subsets. These data suggest that TLRs on T-cells may not function as a primary receptor that controls T-cell function but their distinctive expression profiles on different T-cell subsets suggest plausible immunomodulatory role.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental parasitology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0014489423001868","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
T-cells play a central role in cell-mediated immunity. While activation of T-cells is major histocompatibility-restricted, the Toll-like receptors (TLRs)- a family of proteins that recognize conserved molecular patterns present on the pathogens-are not well-studied for their expression and function in T-cells. As any association of TLR expression profiles with an effector T-cell subset is unknown, we analyze BALB/c mice-derived CD4+ and CD8+ T-cells’ TLR expression profiles. We report: CD4+t-bet+ T-cells are frequent in TLR2LowTLR3HighTLR4Low subpopulation, CD4+GATA3+ T-cells are frequent within the cells with intermediate expression of TLR2, TLR3, TLR4 and TLR11, CD4+FoxP3+ T-cells in TLR2HighTLR3High cells whereas CD4+RORγt + T-cells are frequent in TLR2LowTLR3LowTLR4LowTLR11Low cells. CD4+ effector T-cell subsets may therefore show association with TLRs- TLR3, in particular-expression. In Leishmania donovani infection in BALB/c mice, TLR3 expression on both CD4+ and CD8+ T-cells is reduced. Poly-I:C, a TLR3 ligand, do not have any distinctive effects on the CD4+ effector T-cell subsets. These data suggest that TLRs on T-cells may not function as a primary receptor that controls T-cell function but their distinctive expression profiles on different T-cell subsets suggest plausible immunomodulatory role.
期刊介绍:
Experimental Parasitology emphasizes modern approaches to parasitology, including molecular biology and immunology. The journal features original research papers on the physiological, metabolic, immunologic, biochemical, nutritional, and chemotherapeutic aspects of parasites and host-parasite relationships.