Anass Coulibaly , Abel S. Biguezoton , Delphine M. Hema , Firmin F. Dah , Ignace Sawadogo , Rémy K. Bationo , Moussa Compaoré , Martin Kiendrebeogo , Roger C.H. Nébié
{"title":"Evaluation of synergism in essential oils against the cattle tick Rhipicephalus microplus in Burkina Faso","authors":"Anass Coulibaly , Abel S. Biguezoton , Delphine M. Hema , Firmin F. Dah , Ignace Sawadogo , Rémy K. Bationo , Moussa Compaoré , Martin Kiendrebeogo , Roger C.H. Nébié","doi":"10.1016/j.exppara.2023.108643","DOIUrl":null,"url":null,"abstract":"<div><p>The cattle tick <em>Rhipicephalus microplus</em> affects animal production economically by reducing weight gain and milk production and causing diseases, such as babesiosis and anaplasmosis. Using synthetic acaricides to reduce their incidence has caused the emergence of resistant tick populations. The present study aimed to assess the <em>in vitro</em> acaricidal activity of combinations of essential oils (EOs) from <em>Ocimum americanum</em>, <em>Ocimum gratissimum</em>, and <em>Lippia multiflora</em> against <em>R. microplus</em> larvae. In fact, numerous biological properties have been reported on EOs from these three plants, including acaricidal properties. Hence, a larval immersion test was performed using a population of <em>R. microplus</em> resistant to synthetic acaricides used in Burkina Faso. Results revealed that EO from <em>O. gratissimum</em> was the most effective on <em>R. microplus</em> larvae with LC<sub>50</sub> and LC<sub>90</sub> values at 10.36 and 15.51 mg/mL, respectively. For EO combinations, the most significant synergistic effect was obtained by combination 6 (1/3 <em>O. americanum</em> + 2/3 <em>O. gratissimum</em> +1/6 <em>L. multiflora</em>), with a combination index value of 0.44. All combinations presented dose reduction index >1, indicating a favorable dose reduction. According to the literature, this is the first study to determine the combination effect of EOs from the abovementioned plants in controlling <em>R. microplus</em> activity <em>in vitro.</em> Thus, the combination of these EOs is an alternative to control the resistant populations of invasive cattle ticks.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental parasitology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0014489423001844","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The cattle tick Rhipicephalus microplus affects animal production economically by reducing weight gain and milk production and causing diseases, such as babesiosis and anaplasmosis. Using synthetic acaricides to reduce their incidence has caused the emergence of resistant tick populations. The present study aimed to assess the in vitro acaricidal activity of combinations of essential oils (EOs) from Ocimum americanum, Ocimum gratissimum, and Lippia multiflora against R. microplus larvae. In fact, numerous biological properties have been reported on EOs from these three plants, including acaricidal properties. Hence, a larval immersion test was performed using a population of R. microplus resistant to synthetic acaricides used in Burkina Faso. Results revealed that EO from O. gratissimum was the most effective on R. microplus larvae with LC50 and LC90 values at 10.36 and 15.51 mg/mL, respectively. For EO combinations, the most significant synergistic effect was obtained by combination 6 (1/3 O. americanum + 2/3 O. gratissimum +1/6 L. multiflora), with a combination index value of 0.44. All combinations presented dose reduction index >1, indicating a favorable dose reduction. According to the literature, this is the first study to determine the combination effect of EOs from the abovementioned plants in controlling R. microplus activity in vitro. Thus, the combination of these EOs is an alternative to control the resistant populations of invasive cattle ticks.
期刊介绍:
Experimental Parasitology emphasizes modern approaches to parasitology, including molecular biology and immunology. The journal features original research papers on the physiological, metabolic, immunologic, biochemical, nutritional, and chemotherapeutic aspects of parasites and host-parasite relationships.