Properties of a cell-bound homogeneous murine IgM anti-dextran-V

Immunochemistry Pub Date : 1978-11-01 DOI:10.1016/0161-5890(78)90099-8

Hendrik Pruul, Myron A. Leon

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引用次数: 7

Abstract

O-stearoyl dextran coated sheep erythrocytes (OSD-E) were sensitized with increasing amounts of MOPC 104E IgM to give IgM-OSD-E in antigen excess, in equivalence and in antibody excess. These zones were defined operationally by susceptibility of the cells to complement-mediated lysis, and by the number of free Fab regions on the cell-bound IgM. The free Hab regions were measured by ability to (a) bind soluble dextran and (b) to react with and participate in complement-mediated lysis of bystander OSD-E. In antigen excess, complement-mediated lysis is proportional to cell-bound IgM and there are few free Fab regions. In the equivalence zone, complement-mediated lysis reaches a plateau, while the number of free Fab regions becomes significant. Throughout the zone of antihody excess, complement-mediated lysis diminishes while the number of free Fab region increase exponentially. Blocking these free Fab regions with soluble dextran prevents reactions with bystander OSD-E.

he rate of reaction of IgM-OSD-E, in antibody excess, with bystander OSD-E is proportional to reaction temperature and the extent of reaction is proportional to cell concentration.

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细胞结合均相小鼠IgM抗右旋糖酐- v的性质

O-硬脂酰右旋糖酐包被的绵羊红细胞（OSD-E）用增加量的MOPC 104E IgM致敏，得到抗原过量、等效和抗体过量的IgM-OSD-E。这些区域在操作上通过细胞对补体介导的裂解的易感性以及通过细胞结合的IgM上游离Fab区的数量来定义。通过（a）结合可溶性葡聚糖和（b）与旁观者OSD-E的补体介导的裂解反应并参与其中的能力来测量游离Hab区。在抗原过量的情况下，补体介导的裂解与细胞结合的IgM成比例，并且很少有游离的Fab区域。在等效区，补体介导的裂解达到平稳期，而游离Fab区的数量变得显著。在整个抗抗体过量区域，补体介导的裂解减少，而游离Fab区的数量呈指数级增加。用可溶性葡聚糖阻断这些游离Fab区可防止与旁观者OSD-E的反应。抗体过量的IgM-OSD-E与旁观者OSD-E的反应速率与反应温度成正比，反应程度与细胞浓度成正比。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Immunochemistry

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