Nitrogen and phosphorus addition mediate soil priming effects via affecting microbial stoichiometric balance in an alpine meadow.

Abstract

Priming effect (PE) plays a crucial role in regulating the decomposition of soil organic matter (SOM). Multiple empirical results have shown that nitrogen (N) and phosphorus (P) addition can significantly alter the direction and intensity of PE, which may significantly affect carbon turnover in grasslands, especially in alpine meadows that are sensitive to N and P enrichment. To evaluate the PE responses to N and/or P addition, we conducted an incubation experiment by adding ¹³C-labeled glucose and nutrient additions (+N, +P, and +NP) in soils collected from an alpine meadow. The soils were incubated for 30 days and soil/microbial properties and enzyme activities were measured. Partial correlation and linear regression analyses were then performed to investigate their correlations with PE. The results showed that mean PE intensity among all treatments was 0.61 mg C g^-1 soil or 1.35 (ratio). Nitrogen addition increased PE intensity, which was attributed to the better match between soil resources and microbial demands and enhanced enzyme activities. However, the PE intensity in P-addition soils was lower than that in control soils. This discrepancy may be related to the P-induced decrease of N availability and stronger microbial C/N imbalance. No significant response of PE intensity to NP addition was detected, and this could be explained by the offset of positive N effects and negative P effects on microbial decomposition. In this experiment, N or P addition altered the PE intensity by mediating the match between soil C:N:P ratio and microbial demands, which supported the stoichiometric decomposition hypothesis. Overall, our study highlights the importance of considering the C, N and P coupling in regulating PE, and underscores the need for further investigation into the effects of soil P on microbial activity and SOM decomposition.