In Vitro Levels of Calcium, Phosphate and Alkaline Phosphatase Activity in Media of Rat Osteoblast Grown in the Presence of Various Implant Materials

Abstract

Osteoblast cells from newborn Sprague-Dawley rats were harvested from calvaria. Cells were cultivated in the presence of Cobalt-Chrome (Co-Cr), cpTitanium (Ti), polymethylmethacrylate (PMMA), and wood in artificial media in humidified atmosphere at 37 0 C, 5% CO 2 . Cellular function was monitored over two weeks. Results indicated materials were not significant for Ca 2+ , and PO 4 2- but the time and interaction was significant (p<0.05). For ALP, materials, time and interaction was significant (p<0.05). Wood produced the highest activity for ALP. Electron microscopic studies captured cells growing over all materials and the presence of Ca 2+ in wood samples only.