Determination of brucine in Fengshi Maqian tablet by SPE-HPLC

河北科技大学学报 Pub Date : 2015-04-01 DOI:10.7535/hbkd.2015yx02010

Zetong Wang, Shaomei Wu, Yali Zhang, Yun Ran, Shan Zhang, J. Ha

引用次数: 0

Abstract

To establish the SPE-HPLC method for determining content of brucine in Fengshi Maqian tablet,the cation exchange solid phase extraction column is used to purity the samples. By using PXC cation exchange solid phase extraction( SPE),the HPLC column used in the experiment is C18( 250 mm × 4. 6 mm,5 μm),the mobile phase consists of acetonitrile-0. 01 mol/L sodium heptanesulfonate and 0. 02 mol / L KH2PO4 mixed half and half( adjusting p H to 2. 8 with 10% phosphoric acid)( volume ratio of 21∶ 79),the flow rate is 1. 0 m L / min,the detection wavelength is 262 nm,and the column temperature is 30 ℃. The content of brucine determined by SPE-HPLC method has good linear relationship with the peak areas when their concentration is at 40 ~ 60 μg / m L( r = 0. 999 3). The range of recovery rates of Brucine are 99. 6% ~ 101. 0%( RSD = 1. 04%). This method is simple and accurate with good reproducibility,and it can be used to determine the content of brucine in Fengshi Maqian tablet.

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高效液相色谱法测定风石麻千片中马钱子碱的含量

采用阳离子交换固相萃取柱对样品进行纯化，建立了风石麻千片中马钱子碱含量的SPE-HPLC测定方法。采用PXC阳离子交换固相萃取(SPE)，色谱柱为C18(250 mm × 4)。6 mm,5 μm)，流动相为乙腈-0。01 mol/L庚烷磺酸钠和0。2 mol / L KH2PO4混合一半和一半(调整ph为2)。(体积比为21∶79)，流速为1。0 m L / min，检测波长262 nm，柱温30℃。hplc法测定的马钱子碱含量在40 ~ 60 μg / m L范围内与峰面积呈良好的线性关系(r = 0。3).马钱子碱的回收率范围为99。6% ~ 101。0%(RSD = 1)。04%)。该方法简便、准确，重现性好，可用于风石麻千片中马钱子碱的含量测定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

河北科技大学学报

自引率

0.00%

发文量

3341

审稿时长

16 weeks

期刊介绍： Journal of Hebei University of Science and Technology is a comprehensive academic science and technology journal hosted by Hebei University of Science and Technology, which is openly distributed at home and abroad, published bimonthly at the end of each month, with the domestic uniform serial number CN 13-1225/TS and the international standard serial number ISSN 1008-1542. It is selected as one of the Chinese core journals of Peking University, Chinese core journals of science and technology, RCCSE Chinese core academic journals (A), excellent scientific and technological journals of Chinese colleges and universities, and ‘Top Ten’ journals of Hebei Province. According to the ‘Annual Report on Impact Factor of Chinese Academic Journals (2024 Edition)’ issued by China Knowledge Evaluation Centre in October 2024, the compound impact factor of Journal of Hebei University of Science and Technology is 1.736 (35/146 in subject order), and the influence index (CI) is 336 (62/146 in subject order). Journal of Hebei University of Science and Technology mainly publishes research papers in the fields of [Mechanical, Electronic and Information Science], [Material Science], [Food and Biological Science], [Textile Science], [Civil and Architectural Engineering], [Transportation and Logistics], etc. It also has [National Youth/Regional Science Fund Project Columns ], [Chemistry and Chemical Industry Column], [New Energy and Energy Conservation and Environmental Protection Column], [Data Analysis and Calculation Column] and [Xiongan Green Innovation Column] 5 special columns. Journal of Hebei University of Science and Technology is now published in China Academic Journals Network Publishing Database (China Knowledge), ‘China Science and Technology Paper Statistics Source Journal’ (China Science and Technology Core), Chinese Science and Technology Journal Database (Wipo), Wanfang Database, Changjiang Literature Library, Taiwan Chinese Journal Database, Chinese Journal Database, Chinese Journal Database, Chinese Journal Database, Chinese Journal Database, Chinese Journal Database, and Chinese Journal Database. Database, Changjiang Literature Library, Chinese Electronic Periodical Service in Taiwan - CEPS (CEPS), as well as the American Chemical Abstracts (Web Edition) (CA), the American Ulrich's Journal Guide (Web Edition) (Ulrichsweb), the American EBSCOhost Database (EBSCO), the British Polymer Library (PL), DOAJ (Sweden), HINARI (Switzerland), and JSTChina (Japan Science and Technology Agency).