Features of the in vitro propagation of Pseudotsuga menziesii (Mirb.) Franco

Abstract

The analysis of the works of the scientists engaged in the Pseudotsuga menziesii propagation under the in vitro condition has been made. The findings of the authors’ researches on the propagation of the studied species by micro-cloning are presented. The optimal age of the Pseudotsuga menziesii maternal plants to procure initial plant material has been clarified. The explant decontamination (sterilization) scheme has been deduced from experiments, the nutrient medium composition for the in vitro initiation, multiplication and rooting of regenerated plants, as well as the substrate for the adaptation of the obtained clones to soil conditions, have been selected. The obtained results have shown the prospects of the studied species propagation by the proposed method.