The effect of curcuma longa extract and significance of mordant as an alternative for eosin in H and E: A comparative study.

IF 0.8 4区医学 Q4 PATHOLOGY

Indian Journal of Pathology and Microbiology Pub Date : 2023-10-01 DOI:10.4103/ijpm.ijpm_720_22

M P Rubina, Sukesh, V Soumya, T K Mohammed Safeer, K B Riyas Basheer

{"title":"The effect of curcuma longa extract and significance of mordant as an alternative for eosin in H and E: A comparative study.","authors":"M P Rubina, Sukesh, V Soumya, T K Mohammed Safeer, K B Riyas Basheer","doi":"10.4103/ijpm.ijpm_720_22","DOIUrl":null,"url":null,"abstract":"Background and aim: Hematoxylin and Eosin (H and E) staining is a common and routine staining method used in Histopathology. In origin, Hematoxylin is natural and Eosin is a synthetic dye. Synthetic dyes are widely accepted due to its staining efficiency. However, due to synthetic and toxic properties, natural alternatives have gained importance. In this present study, Curcuma Longa rhizome (Turmeric) extract with and without mordant are examined as a natural substitute for Eosin in H and E staining. This study assessed the staining quality of Turmeric (H and T) compared to Eosin (H and E) and the significance of mordant in H and T staining.Materials and methods: A comparative study is conducted in a Histopathology laboratory in a tertiary care hospital. Five tissue specimens were collected and stained with H and T with mordant, H and T without mordant, and conventional H and E stain. Two independent pathologists examined the quality of each stain. The results are graded as excellent, good, and poor. These results are statistically compared and analyzed.Result: Staining quality of Turmeric and Eosin are comparable to each other. At the same time, Turmeric gives yellow color instead of pink in Eosin. The result also shows that the addition of mordant in Turmeric significantly improves the staining quality.Interpretation and conclusion: Turmeric with mordant can be used as an alternative stain to synthetic Eosin in H and E staining.","PeriodicalId":13488,"journal":{"name":"Indian Journal of Pathology and Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Pathology and Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4103/ijpm.ijpm_720_22","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PATHOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background and aim: Hematoxylin and Eosin (H and E) staining is a common and routine staining method used in Histopathology. In origin, Hematoxylin is natural and Eosin is a synthetic dye. Synthetic dyes are widely accepted due to its staining efficiency. However, due to synthetic and toxic properties, natural alternatives have gained importance. In this present study, Curcuma Longa rhizome (Turmeric) extract with and without mordant are examined as a natural substitute for Eosin in H and E staining. This study assessed the staining quality of Turmeric (H and T) compared to Eosin (H and E) and the significance of mordant in H and T staining.

Materials and methods: A comparative study is conducted in a Histopathology laboratory in a tertiary care hospital. Five tissue specimens were collected and stained with H and T with mordant, H and T without mordant, and conventional H and E stain. Two independent pathologists examined the quality of each stain. The results are graded as excellent, good, and poor. These results are statistically compared and analyzed.

Result: Staining quality of Turmeric and Eosin are comparable to each other. At the same time, Turmeric gives yellow color instead of pink in Eosin. The result also shows that the addition of mordant in Turmeric significantly improves the staining quality.

Interpretation and conclusion: Turmeric with mordant can be used as an alternative stain to synthetic Eosin in H and E staining.

查看原文本刊更多论文

莪术提取物在 H 和 E 中替代曙红的效果和媒染剂的意义：一项比较研究。

背景和目的：苏木精和伊红（H 和 E）染色是组织病理学常用的常规染色方法。血色素是天然的，而伊红是合成染料。合成染料因其染色效率高而被广泛接受。然而，由于合成染料的毒性，天然替代品越来越受到重视。在本研究中，姜黄提取物（含媒染剂和不含媒染剂）在 H 和 E 染色中可作为伊红的天然替代品。本研究评估了姜黄（H 和 T）与伊红（H 和 E）相比的染色质量，以及媒染剂在 H 和 T 染色中的重要性：比较研究在一家三级医院的组织病理学实验室进行。收集了五份组织标本，分别用带媒染剂的 H 和 T 染色法、不带媒染剂的 H 和 T 染色法以及传统的 H 和 E 染色法进行染色。两名独立病理学家对每种染色的质量进行了检查。结果分为优、良、差三个等级。对这些结果进行统计比较和分析：结果：姜黄和伊红的染色质量相当。同时，姜黄呈现黄色，而不是伊红的粉红色。结果还表明，在姜黄中添加媒染剂可显著提高染色质量：解释与结论：在 H 和 E 染色中，添加媒染剂的姜黄可用作合成伊红的替代染色剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Indian Journal of Pathology and Microbiology PATHOLOGY-

CiteScore

1.20

自引率

0.00%

发文量

422

审稿时长

1 months

期刊介绍： The journal will cover studies related to pathology including morbid anatomy, surgical pathology, clinical pathology, diagnostic cytopathology including gynecologic cytology and aspiration cytology, hematology including immuno-hematology and medical microbiology. The journal gives preference to clinically oriented studies over experimental and animal studies. The Journal would publish peer-reviewed original research papers, case reports, systematic reviews, meta-analysis, letters to the editor and brief communications. Review articles on current topics usually are invited by the editor.