Detection of Babesia spp by using of Acridine Orange and Giemsa Stain with Study of its Effect on Blood Picture in Local Breed Cows.

Abstract

This study was conducted to diagnose of Babesia spp by using fluorescent microscope and light microscope, the study also included evaluating of Hemogram (RBC, HB, PCV, MCV, MCHC, Total Leukocyte Count, Differential Leukocyte Count) in cows infected with Babesia spp which cause piroplasmosis. The study included examination of 60 local breed Cows from many districts of Mosul city from the beginning of February 2018 until the end of July 2018, 17 were clinically infected with piroplasmosis and the infection was confirmed by using Giemsa and Acridine orange staining blood smears, and 10 cows were clinically intact and served as control. the results indicated that the percentage of the infection with Babesia spp was 28.33%, and the percentage of parasitemia ranged between (5-23%) with mean of (12.57%). The Results showed a statistical significant decrease (P<0.05) in RBC, Hb, and PCV values of diseased animals, Normocytic Normochromic type of anemia was found, the results also indicated a significant decrease in total Leukocyte count and neutrophil, with significant increase in lymphocytes number. the results also indicated non-significant changes in Eosinophil, Basophil and Monocyte numbers.