A review of molecular biology detection methods for human adenovirus

IF 1.1 Q4 BIOPHYSICS
N. Datta
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引用次数: 0

Abstract

In humans particularly in children, adenovirus is one of the most common viruses that cause respiratory illnesses. Knowing how to detect adenovirus proficiently and rapidly will help reinforce surveillance of adenovirus infections, detect epidemic situations in real-time, and understand the trend of virus epidemics, which will allow effective actions to be taken quickly. The rapid detection of antiviral antibodies or viral antigens in clinical samples can be achieved by molecular diagnostic techniques like PCR, Real-Time PCR, LAMP, mPCR-RLB, PCR-ELISA, Tem-PCR, Gene Chip, and so on. Some of the molecular diagnostic methods are relatively economical, exceedingly sensitive and explicit. There are several commercially accessible molecular diagnostic techniques that enable their use in clinical laboratories all over the world. In this review, the principles, characteristics, and applications of molecular biology surveillance methods commonly used in labs and clinics for the detection of human adenoviruses are examined and highlighted.
人腺病毒分子生物学检测方法综述
在人类,特别是儿童中,腺病毒是引起呼吸道疾病的最常见病毒之一。掌握如何熟练、快速地检测腺病毒,有助于加强对腺病毒感染的监测,实时发现疫情,了解病毒流行趋势,从而迅速采取有效措施。PCR、Real-Time PCR、LAMP、mPCR-RLB、PCR- elisa、Tem-PCR、Gene Chip等分子诊断技术可实现临床样品中抗病毒抗体或病毒抗原的快速检测。一些分子诊断方法相对经济,非常敏感和明确。有几种商业上可获得的分子诊断技术,使它们能够在世界各地的临床实验室中使用。本文综述了实验室和临床常用的分子生物学监测方法的原理、特点和应用。
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来源期刊
AIMS Biophysics
AIMS Biophysics BIOPHYSICS-
CiteScore
2.40
自引率
20.00%
发文量
16
审稿时长
8 weeks
期刊介绍: AIMS Biophysics is an international Open Access journal devoted to publishing peer-reviewed, high quality, original papers in the field of biophysics. We publish the following article types: original research articles, reviews, editorials, letters, and conference reports. AIMS Biophysics welcomes, but not limited to, the papers from the following topics: · Structural biology · Biophysical technology · Bioenergetics · Membrane biophysics · Cellular Biophysics · Electrophysiology · Neuro-Biophysics · Biomechanics · Systems biology
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