Regenerative processes during model gastric ulcer in laboratory rats when using Humilid

Abstract

A gastric ulcer caused by non-steroidal anti-inflammatory drugs is the second most common after Helicobacter pylori in both humane and veterinary medicine. The urgent need to find alternative drugs that had a minimum number of side effects led to the use of a model pathology that mimics a stomach ulcer. The article presents the results of a study of the effect of Humilid on the healing processes of a model gastric ulcer induced by Ibuprofen, a non-steroidal anti-inflammatory drug. The study lasted 21 days. It was found that Ibuprofen at a dose of 400 mg/kg on the 3rd day of the experiment caused damage to the gastric mucosa, namely an ulcer, demonstrated by both macroscopic and histological examination. In the animals of the group where Humilide (5 mg/kg) was used against the background of Ibuprofen-induced ulcers, on day 21 of the experiment, the ulcer score (Ulcer score) was 94% lower (P <0.001) compared to the ulcer score of the same group by 3 - e day of the experiment. And in animals of the group where the ulcer was formed, but Gumilid was not used, the difference in ulcer scores on day 21 of the experiment was 81% (P <0.001) compared to the ulcer score in rats receiving Humilid. A histological study showed that in animals of the group receiving Humilid, such indicators as desquamation of the epithelium, erosive and ulcerative destruction, stromal oedema and inflammatory infiltration were 56%, 62%, 30% and 60% (P <0.001) lower, respectively, compared to with a group of model pathology, where it was not received. Thus, it was found that with Ibuprofen-induced gastric ulcer, including Humilide in the general diet of animals in the experimental group, accelerates and improves regenerative processes, namely, the healing of stomach ulcers.