Characterisation and Expression Analysis of Sox9 in the Multiocellated Racerunner, Eremias multiocellata

IF 1.2 3区生物学 Q2 ZOOLOGY

Asian Herpetological Research Pub Date : 2014-06-25 DOI:10.3724/SP.J.1245.2014.00104

Y. Xin, Hh Wang, Xl Tang, S. Lu, WX（李文新） Li, Y. Zhang, S. Liang, Qiang Chen

引用次数: 2

Abstract

Sox9 is an important member of Sox family which is involved in a variety of developmental processes including sex determination and gonadal differentiation. The cDNA of Sox9 from multiocellated racerunner E. multiocellata was cloned using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The sequence contains a 1497 bp open reading frame, which encodes a 498 amino acid protein with a predicted molecular weight of 55.45 kDa. EmSox9 displays high similarity to those of reptiles, and shows an overall amino acid identity of >82%. We also investigated the tissue-specific expression of EmSox9 mRNA by real-time quantitative PCR. Sox9 mRNA is present in brain, heart, liver, kidney, gonads and muscle tissues of adult E. multiocellata, with the highest expression in brain and testis. The results indicate that Sox9 may play important roles in some tissues during E. multiocellata neural and gonadal development.

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多细胞赛跑动物Sox9基因的鉴定及表达分析

Sox9是Sox家族的重要成员，参与多种发育过程，包括性别决定和性腺分化。利用逆转录聚合酶链反应(RT-PCR)和cDNA末端快速扩增技术(RACE)克隆了多细胞赛跑鼠Sox9基因的cDNA。该序列包含一个1497 bp的开放阅读框，编码一个498个氨基酸的蛋白，预测分子量为55.45 kDa。EmSox9与爬行动物具有较高的相似性，氨基酸同源性为>82%。我们还通过实时定量PCR研究了EmSox9 mRNA的组织特异性表达。Sox9 mRNA在成虫的脑、心、肝、肾、性腺和肌肉组织中均有表达，其中脑和睾丸表达量最高。结果表明，Sox9可能在多细胞棘球蚴神经和性腺发育过程中发挥重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Asian Herpetological Research ZOOLOGY-

CiteScore

2.80

自引率

7.10%

发文量

441

审稿时长

>12 weeks

期刊介绍： AHR aims to provide a forum for herpetologists and related scientists interested in conducting international academic exchanges and joint studies, and a platform for introducing their newly made scientific and technological data, and publishing their research results and achievements in the world, but focusing on the Asian-Pacific Region. The principal criteria of AHR for acceptance of articles for publication are the quality and significance of the research, breadth of interest of the work to the readership, and the clarity and effectiveness of communication. AHR welcomes submission of manuscripts from authors in all countries of the world, though with a focus on the herpetological studies in the Asian and Pacific Region, including major articles, shorter communications and review articles.

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