Microsatellite analysis of population genetic diversity in Triplophysa xiangxiensis

Q4 Environmental Science

水产学报 Pub Date : 2013-01-01 DOI:10.3724/SP.J.1231.2013.37800

Yanhong Yao, Lingfu Kong, Dengqiang Wang, Wenhui He, Li He, L. Yu

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引用次数: 2

Abstract

The cavefish Triptophysa xiangxiensis is a species of loach belonging to family Cobitidae,order Cypriniformes.It is endemic to Longshan County of western Hunan Province of China.In this study,a total of 103 individuals from three T.xiangxiensis populations obtained from three caves in Wulongshan Mountain were studied using 16 pairs of microsatellite markers.Using polymorphism information content(PIC),mean heterozygosity(H),number of effective alleles and F-statistics,the genetic diversity and genetic differentiation were evaluated.A total of 83 different alleles were detected in all examined loci.The number of alleles ranged from 3 to 8,with an average number of about 5 per locus.The observed(Ho)and expected heterozygosity(He)ranged from 0.362 5 to 0.946 5 and from 0.538 6 to 0.906 5,respectively.The polymorphism information content for these three populations were 0.263 2,0.231 3,0.303 5.The results from this study indicated that two of the selected 16 microsatellite loci were high polymorphic and two loci were low polymorphic,the other twelve microsatellite loci showed moderate polymorphic.The analysis of molecular variance(AMOVA)indicated that almost majority of the variance in the T.xiangxiensis was within stocks(92.84%),and 7.16% was among stocks.The result of AMOVA,F-statistics,Nei's genetic distance and genetic identity indicated that genetic difference was relatively small and genetic differentiation was low,with high genetic identity between both populations.The information obtained in this study will contribute to the conservation of this endangered T.xiangxiensis species.

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湘西三树居群遗传多样性的微卫星分析

湘西洞鱼是泥鳅科鲤形目泥鳅的一种。它是中国湖南省西部龙山县的特有种。本研究利用16对微卫星标记对武龙山3个洞穴中3个湘西猿猴种群的103个个体进行了研究。利用多态性信息含量(PIC)、平均杂合度(H)、有效等位基因数和f统计量，对其遗传多样性和遗传分化进行了评价。在所有检测的位点中共检测到83种不同的等位基因。等位基因数量在3 ~ 8个之间，平均每个位点5个左右。观察到的杂合度(Ho)和期望杂合度(He)分别在0.362 ~ 0.946 5和0.538 ~ 0.9065之间。3个群体的多态性信息含量分别为0.263 2、0.231 3、0.303 5。结果表明，16个微卫星位点中2个为高多态性位点，2个为低多态性位点，其余12个微卫星位点为中等多态性位点。分子方差分析(AMOVA)表明，湘西柽柳种群的变异主要发生在种群内(92.84%)，种群间(7.16%)。AMOVA、f统计、Nei’s遗传距离和遗传同一性分析结果表明，两居群遗传差异较小，遗传分化程度较低，遗传同一性较高。本研究所得资料将为湘西石竹濒危物种的保护提供参考。

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来源期刊

水产学报 Environmental Science-Management, Monitoring, Policy and Law

CiteScore

1.40

自引率

0.00%

发文量

5213

期刊介绍： "Fisheries of" mainly reflects the results of scientific research and development of the direction of aquaculture for domestic and foreign academic exchanges Fisheries Service. Mainly basic research published in Fisheries, aquaculture and proliferation of fishing waters environmental protection, preservation of aquatic products processing and utilization, fishing equipment, and other aspects of mechanical papers, research briefings and reviewed.