Confocal laser speckle autocorrelation imaging of dynamic flow in microvasculature

IF 15.3 1区物理与天体物理 Q1 OPTICS

Opto-Electronic Advances Pub Date : 2022-01-01 DOI:10.29026/oea.2022.210045

E. Du, Shuhao Shen, Anqi Qiu, Nanguang Chen

引用次数: 9

Abstract

Laser speckle imaging has been widely used for in-vivo visualization of blood perfusion in biological tissues. However, existing laser speckle imaging techniques suffer from limited quantification accuracy and spatial resolution. Here we report a novel design and implementation of a powerful laser speckle imaging platform to solve the two critical limitations. The core technique of our platform is a combination of line scan confocal microscopy with laser speckle autocorrelation imaging, which is termed Line Scan Laser Speckle Autocorrelation Imaging (LS-LSAI). The technical advantages of LS-LSAI include high spatial resolution (~4.4 μm) for visualizing and quantifying blood flow in microvessels, as well as video-rate imaging speed for tracing dynamic flow

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微血管动态血流的激光共聚焦散斑自相关成像

激光散斑成像已广泛应用于生物组织血液灌注的体内可视化。然而，现有的激光散斑成像技术存在定量精度和空间分辨率有限的问题。在这里，我们报告了一个新颖的设计和实现一个强大的激光散斑成像平台，以解决这两个关键的限制。我们平台的核心技术是线扫描共聚焦显微镜与激光散斑自相关成像的结合，称为线扫描激光散斑自相关成像(LS-LSAI)。LS-LSAI的技术优势包括高空间分辨率(~4.4 μm)，用于微血管血流的可视化和量化，以及视频速率成像速度，用于跟踪动态血流

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Opto-Electronic Advances OPTICS-

CiteScore

19.30

自引率

7.10%

发文量

128

期刊介绍： Opto-Electronic Advances (OEA) is a distinguished scientific journal that has made significant strides since its inception in March 2018. Here's a collated summary of its key features and accomplishments: Impact Factor and Ranking: OEA boasts an impressive Impact Factor of 14.1, which positions it within the Q1 quartiles of the Optics category. This high ranking indicates that the journal is among the top 25% of its field in terms of citation impact. Open Access and Peer Review: As an open access journal, OEA ensures that research findings are freely available to the global scientific community, promoting wider dissemination and collaboration. It upholds rigorous academic standards through a peer review process, ensuring the quality and integrity of the published research. Database Indexing: OEA's content is indexed in several prestigious databases, including the Science Citation Index (SCI), Engineering Index (EI), Scopus, Chemical Abstracts (CA), and the Index to Chinese Periodical Articles (ICI). This broad indexing facilitates easy access to the journal's articles by researchers worldwide.