In silico and pepscan analysis of African swine fever virus p54 protein

IF 0.6 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria Brno Pub Date : 2023-01-01 DOI:10.2754/avb202392010047

Dagmar Břínek Kolařová, Zuzana Úlehlová, Lucie Janíček Hrubá, Klára Klíčová, V. Celer

引用次数: 0

Abstract

Due to the great importance and economic impact of African swine fever, great emphasis is placed on the diagnosis of its causative agent and the development of new types of vaccines. However, a prerequisite for the shift in these areas is a more thorough understanding of the structure and characteristics of virus proteins. The aim of this work was to analyze the location of B-cell epitopes in the structure of the African swine fever virus p54 protein, combining the results of in silico and pepscan analysis. In silico analysis revealed the presence of potential B-cell epitopes primarily in the C-terminal part of the protein, pepscan technique confirmed these immunogenic regions between amino acids 24-61 and 151-182.

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非洲猪瘟病毒p54蛋白的计算机和胃扫描分析

由于非洲猪瘟的重要性和经济影响，因此高度重视其病原体的诊断和新型疫苗的开发。然而，在这些领域发生转变的先决条件是对病毒蛋白质的结构和特征有更全面的了解。本工作的目的是结合硅片和胃扫描分析的结果，分析非洲猪瘟病毒p54蛋白结构中b细胞表位的位置。硅分析显示潜在的b细胞表位主要存在于蛋白质的c端，pepscan技术证实了这些氨基酸24-61和151-182之间的免疫原性区域。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Veterinaria Brno 农林科学-兽医学

CiteScore

1.00

自引率

33.30%

发文量

审稿时长

18-36 weeks

期刊介绍： ACTA VETERINARIA BRNO is a scientific journal of the University of Veterinary and Pharmaceutical Sciences in Brno, Czech Republic. The scientific journal Acta Veterinaria Brno is dedicated to the publication of original research findings and clinical observations in veterinary and biomedical sciences. Original scientific research articles reporting new and substantial contribution to veterinary science and original methods that have not been submitted for publication elsewhere are considered for publication. A written statement to this effect should accompany the manuscript, along with approval for publication by the author´s head of department. The authors bear full responsibility for the contents of their contribution. Book reviews are published, too.