Genetic Diversity of Loquat Accessions in Japan as Assessed by SSR Markers

Journal of The Japanese Society for Horticultural Science Pub Date : 2013-04-01 DOI:10.2503/JJSHS1.82.131

S. Fukuda, C. Nishitani, N. Hiehata, Y. Tominaga, H. Nesumi, Toshiya Yamamoto

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引用次数: 10

Abstract

The genetic variation of loquat (Eriobotrya japonica (Thunb.) Lindl.) was characterized by SSR markers developed from apple and pear, using 94 loquat accessions in Japan. Fourteen of the 24 SSR markers derived from apple could successfully produce amplified bands in loquat, whereas 10 of the 24 SSR markers derived from pear could generate amplified bands. Nine SSR markers were chosen for evaluation of the genetic diversity among 94 loquat accessions, including 61 cultivars from Japan and other countries and 33 natively grown accessions collected around Japan. A phenogram constructed using the unweighted pair-group method with arithmetic averages based on similarities between genotypes revealed two major groups. One group consisted mainly of cultivars from Japan and other countries, whereas the other group included only natively grown accessions. Some synonyms or mutants were found showing identical SSR genotypes. These results show that SSR markers can be utilized as reliable tools for genetic identification in loquat. The origins of current loquat cultivars in Japan are also discussed.

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利用SSR标记评价日本枇杷种质资源的遗传多样性

枇杷(Eriobotrya japonica)的遗传变异利用日本94份枇杷材料，对苹果和梨的SSR标记进行了鉴定。来自苹果的24个SSR标记中有14个能在枇杷上产生扩增带，而来自梨的24个SSR标记中有10个能在枇杷上产生扩增带。选择9个SSR标记，对94份枇杷材料进行遗传多样性评价，其中包括61份来自日本和其他国家的枇杷品种和33份来自日本各地的本土枇杷材料。利用基于基因型相似性的算术平均值的非加权配对组方法构建的表型图揭示了两个主要群体。一组主要由来自日本和其他国家的品种组成，而另一组仅包括本地种植的品种。发现一些同义或突变体具有相同的SSR基因型。这些结果表明，SSR标记可以作为枇杷遗传鉴定的可靠工具。本文还讨论了日本枇杷品种的起源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of The Japanese Society for Horticultural Science 农林科学-园艺

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审稿时长

>36 weeks