Transplantation of DBA/2 Mouse Corneas in BALB/c Recipients SignificantlyDelays Graft Rejection Compared with C3H Grafts and FacilitatesStudies on Gene Transfer

The open transplantation journal Pub Date : 2008-05-14 DOI:10.2174/1874418400802010009

R. Ignatius, E. Zhang, Felix Schulte, M. Schroff, U. Rüschendorf, A. Müller, B. Wittig, F. Hoffmann

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Abstract

To study the impact of gene transfer in experimental corneal transplantation, grafts should reliably be rejected but after a time span, which allows multiple interventions. We were interested in delaying transplant survival time through MHC-matching of donor and recipient animals and in omitting local dexamethasone treatment, which is laborious and could potentially interfere with the expression of administered DNA. Rejection of DBA/2 (H-2d) corneas by BALB/c (H- 2d) recipients occurred significantly delayed compared with C3H (H-2k) grafts (25.0 ± 5.6 vs. 14.5 ± 4.1 days, p=0.038), and correlated with the expression of allospecific T cell-proliferation and IFN-￻ secretion in draining lymph nodes. Gene gun treatment with IL-4/CTLA-4 DNA MIDGE TM vectors in the lower lid of recipients significantly prolonged survival of DBA/2 corneas in BALB/c mice. This refined and dexamethasone-free protocol may be advantageous for the develop- ment of novel treatment strategies, e.g., local gene transfer, in corneal transplantation.

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与C3H相比，BALB/c受体DBA/2小鼠角膜移植可显著延缓移植排斥反应，促进基因转移研究

为了研究基因转移对实验性角膜移植的影响，移植物应该可靠地排斥，但要经过一段时间，这允许多种干预。我们感兴趣的是通过供体和受体动物的mhc匹配来延迟移植存活时间，并省略局部地塞米松治疗，这是费力的，可能会干扰给药DNA的表达。与C3H (H-2k)相比，BALB/c (H-2d)受体对DBA/2 (H-2d)角膜的排斥反应发生明显延迟(25.0±5.6 vs 14.5±4.1天，p=0.038)，并与引流淋巴结中异体特异性T细胞增殖表达和IFN-￻分泌有关。受体下眼睑IL-4/CTLA-4 DNA MIDGE TM载体基因枪处理可显著延长BALB/c小鼠DBA/2角膜的生存期。这种改进的和不含地塞米松的方案可能有利于开发新的治疗策略，例如角膜移植中的局部基因转移。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The open transplantation journal

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