In vitro Anthelmintic Activity of Ethanolic Extracts of Camellia sinensis L. and Albizia lebbeck L. against Haemonchus contortus

Abstract

| Helminths are familiar as a major problem to livestock production. Helminths cause multiple infections in animals of significant economic importance. Haemonchus contortus, (helminths worm), is highly infectious parasite of small ruminants, and is a major cause of diseases and significant mortality in various cattle, sheep, deer, buffalo and goat, etc. Various synthetic anthelmintics have been used, on wide scale, to control the helminths infections.But the parasite worms have developed multiple resistances against the synthetic anthelmintics. Hence, various biological controls, vaccines and local medicinal plants are being explored to control the parasitic worms. The aim of this study was to evaluate the anthelmintic potential of two local plants, Albizia lebbeck L. and Camellia sinensis L against H. contortus using Adult Motility Assay (AMA). Crude Ethanolic Extracts (CEE)of dried leaves of A. lebbeck L. and whole plant of C. sinensis L. were prepared by cold maceration method. The anthelmintic activity was analyzed by adult motility assay (AMA). Levamisole was used as positive control to compare the efficacy of CEE of both plants. Adult worms were treated with different concentrations i.e., 2, 4, 6 and 8 mg/ml of ethanolic extract and motility of the worms was examined after every two to eight hours. Both A. lebbeck and C. sinensis exhibited 88% and 95% mortality at 6 & 8 mg/ml after 8 hrs of treatment. In conclusion, the ethanolic extracts of A. lebbeck L. and C. sinensis L. showed in vitro anthelmintic activity against H. contortus at concentrations tested. Article History Received: November 11, 2018 Revised: February 01, 2019 Accepted: February 05, 2019 Published: March 01, 2019 Authors’ Contributions SZ performed experimental work. AH designed and supervised the research. AQ reviewed the literature. MM complied the data and helped in manuscript writing. ML provided lab facilities and assisted in the experimental work.