Impact of DEHP Toxicity on Testis of Rat Leading to Disruption of Testis Histology

Abstract

Di (2-ethyl hexyl) Phthalate (DEHP) is one of the plasticizers widely used in the manufacturing of plastics to make it flexible and durable. Present study is focussed to observe the deleterious effects of DEHP on male reproductive system of animals. For this, 1000 mg/kg body wt. of DEHP was administered to different groups of male Wistar rat for 2, 4, 6 and 8 weeks. After each interval, rats were sacrificed and histological alterations in testis of rats were observed. On hormonal assay, testosterone level decreased significantly in DEHP exposed groups. The histological structure of the testis was also observed to be disrupted significantly with increasing duration of DEHP exposure. Organisation of seminiferous tubule was found distorted and disoriented showing large gaps between them along with degenerated epithelium. Evident changes in morphology of spermatozoa were seen with gradual loss of head and tail structure. Decrease in the number of Leydig cells and sertoli cells were also found suggesting DEHP as a potent toxicant for male reproductive system.