Molecularly Imprinted Polymer Solid Phase Extraction followed by High-Performance Liquid Chromatography as an Efficient and Sensitive Technique for Determination of Meropenem in Human Plasma and Urine

IF 0.8 4区化学 Q4 CHEMISTRY, MULTIDISCIPLINARY

South African Journal of Chemistry-Suid-Afrikaanse Tydskrif Vir Chemie Pub Date : 2019-01-01 DOI:10.17159/0379-4350/2019/V72A5

Hamoon Sami Amlashi, Ameneh Porgham Daryasari, M. Soleimani

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引用次数: 3

Abstract

A new and selective sorbent for molecularly imprinted polymer solid-phase extraction (MIP-SPE) was prepared to extract meropenem from plasma and urine samples. The extracted analyte was analyzed by high-performance liquid chromatography (HPLC) coupled with photodiode array detection. Methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linker, azobisisobutyronitrile (AIBN) as an initiator and meropenem as a template molecule were used for the MIP preparation. Imprinted meropenem molecule was removed from the polymeric structure using acetonitrile in water (1:9, v/v), as the eluent solvent. Under the optimized conditions, the limit of detection (LOD) and limit of quantification (LOQ) were 35 and 120 μg L, respectively. The developed MIP-SPE method demonstrates that it could be applied for the determination of meropenem in plasma and urine samples.

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来源期刊

South African Journal of Chemistry-Suid-Afrikaanse Tydskrif Vir Chemie 化学-化学综合

CiteScore

3.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Original work in all branches of chemistry is published in the South African Journal of Chemistry. Contributions in English may take the form of papers, short communications, or critical reviews.