Voltammetric and sequential injection amperometric determination of B group vitamins on electrodes modified with binary systems based on oxides of manganese, bismuth and gold

Abstract

It was found that oxides of manganese (MnOx), bismuth (ВiOx), gold (AuOx), and ВiOx–MnOx and MnOx–AuOx binary systems electrodeposited on the surface of planar carbon electrodes (SPE) exhibited catalytic activity during electrooxidation of thiamine, riboflavin, pyridoxine and cyanocobalamin in acidic media. Immobilization of the binary systems on the surface of the SPE working electrode allowed to achieve a joint selective voltammetric determination of two-component systems of the vitamins considered: riboflavin and pyridoxine on SPE with BiOx–MnOx (ВiOx–MnOx–SPE) and MnOx–AuOx (MnOx–AuOx–SPE); and riboflavin and thiamine or riboflavin and cyanocobalamin on MnOx–AuOx–SPE. The calibration dependences obtained for the considered vitamins using voltammetry on BiOx–MnOx–SPE and MnOx–AuOx–SPE electrodes were observed in the range from 1×10–6 to 1×10–3 M. Detection limit of thiamine, pyridoxine and cyanocobalamin was decreased by an order of magnitude compare to static conditions in a sequential injection system (SI-system). To implement two- and three-component analysis of biologically active additives (BAA) and drugs in the SI-system, the surface of the working electrode modified with MnOx–AuOx binary system was covered with poly-2-vinylpyridine (PVP) or Nafion (Nf) polymer films. SPE with two working electrodes (DSPE) modifies with MnOx–AuOx were used in the two-component analysis of BAA samples. PVP film was deposited on the electrode for thiamine determination and Nf film was deposited on the electrode for pyridoxine determination. Combination of SPE and DSPE with polymer composites containing MnOx–AuOx binary system and PVP or Nf in a two-detector SI-system made it possible to implement a three-component analysis of drugs containing thiamine, pyridoxine and cyanocobalamin. Excipients present in them did not interfere with the results of the determination. Application of the proposed SI-system scheme increased selectivity, speed and productivity of the analysis to 540 determinations per hour.