Effect of toothpaste with high fluoride concentration on remineralization of dental substrate with artificial caries

Diego Felipe Mardegan Gonçalves, Eri Omoto, Yasmine Parischi MUSA ALI, A. Briso, T. Fagundes
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Abstract

Abstract Introduction fluoride toothpaste is widely used by the population. Objective dentifrices with a high concentration of sodium fluoride and the presence of tricalcium phosphate were developed, providing additional protection to patients vulnerable to the development of caries. This study aimed to evaluate the influence of different dentifrices on the surface and internal hardness and enamel wear after artificial caries, pH cycling, and toothbrushing. Material and method enamel blocks (4x4 mm) were obtained from 20 bovine teeth. The specimens were submitted to artificial caries lesion and then to pH cycling and brushing according to the dentifrice: Colgate Total 12 and Clinpro + Tricalcium Phosphate. Result after this period, they were submitted to analysis of superficial and internal hardness and profilometry. Scanning Electron Microscopy (SEM) was performed. The data obtained were evaluated by one-way Analysis of Variance (ANOVA) to evaluate the mineral recovery and profilometry, and two-way repeated measures ANOVA to evaluate the superficial and internal hardness. Conclusion analyzing the surface hardness, after caries induction, there was a significant reduction in hardness that was partially recovered regardless of the dentifrice used. For internal hardness, Clinpro obtained lower values on the control side and up to 90 micrometers on the test side. As for profilometry, Colgate Total 12 showed greater wear when compared to Clinpro. It was concluded that Clinpro promoted lower internal hardness, however, Colgate Total 12 resulted in greater surface wear of tooth enamel.
高氟牙膏对人工龋牙基质再矿化的影响
含氟牙膏是人们广泛使用的牙膏。目的研制出含有高浓度氟化钠和磷酸三钙的牙膏,为易患龋齿的患者提供额外的保护。本研究旨在评估人工龋齿、pH循环和刷牙后不同牙釉质对牙釉质表面和内部硬度及磨损的影响。材料和方法从20颗牛牙中获得4 × 4 mm的牙釉质块。将标本提交人工龋损,然后根据牙膏:高露洁Total 12和克林普+磷酸三钙进行pH循环和刷牙。结果在这段时间后,他们提交了表面和内部硬度和轮廓分析。扫描电镜(SEM)观察。所得数据采用单因素方差分析(ANOVA)评价矿物回收率和轮廓度,双因素重复测量方差分析(ANOVA)评价表面硬度和内部硬度。结论通过对表面硬度的分析,无论使用何种牙釉质,诱发龋病后,牙釉质硬度均有明显下降,且有部分恢复。对于内部硬度,Clinpro在控制侧获得较低的值,在测试侧获得高达90微米的值。在轮廓测量方面,与Clinpro相比,高露洁Total 12显示出更大的磨损。结果表明,Clinpro可降低牙釉质的内部硬度,而高露洁Total 12可导致牙釉质表面磨损。
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