Identification of single nucleotide polymorphism c.957A>C of PLAG1 gene and its association with growth traits in Bali cattle

IF 0.3 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of the Indonesian Tropical Animal Agriculture Pub Date : 2021-07-10 DOI:10.14710/jitaa.46.3.199-208

I. G. Putra, D. A. Sari, S. Rachmawati, R. Oktaviani, R. R. Noor, J. Jakaria

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引用次数: 1

Abstract

The PLAG1 gene is one of the genes that affect the growth traits located on chromosome 14 in cattle. This study aims to obtain SNP of the PLAG1 gene in exon 1 and exon 2 and their association with growth traits in Bali cattle. The number of samples used was 52 samples of Bali cattle, 10 samples of Peranakan Ongole (PO), and 8 samples of Limousine cattle. Identification of SNPs PLAG1 gene was analyzed by direct sequencing method and genotyping of selected SNPs was carried out using PCR-RFLP. Association of genotypes of SNP c.957A>C with growth using t-test. There were 7 SNPs in exon 2 of the PLAG1 gene, namely SNP c.339A>G, c.489C>T, c.795A>G, c.957A>C, c.1023C>T, c.1056A>G, and c.1353A>G. SNP c.957A>C was validated by PCR-RFLP using TaqI enzyme and obtained three genotypes, namely genotypes AA, AC, and CC with allele frequency A and C, respec-tively 0.10 and 0.90 in Bali cattle, while in PO and Limousine cattle were monomorphic. Genotype association of SNP c.957A>C PLAG1 gene were not associated with birth weight (BW0), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365), yearling weight at 730 days of age (YW730), and average daily gain (ADG). SNP c.957A>C as a specific SNP for Bali cattle needs to be investigated in further research as a candidate marker for growth traits in Bali cattle.

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巴厘牛PLAG1基因单核苷酸多态性C . 957a >C的鉴定及其与生长性状的关系

PLAG1基因是影响牛生长性状的基因之一，位于14号染色体上。本研究旨在获得巴厘牛PLAG1基因外显子1和外显子2的SNP及其与生长性状的关系。样本数量为:巴厘岛牛52个，土生牛10个，豪华牛8个。采用直接测序法鉴定snp的PLAG1基因，采用PCR-RFLP对所选snp进行基因分型。用t检验分析SNP C . 957a >C基因型与生长的关系。PLAG1基因外显子2共有7个SNP，分别为C . 339a >G、C . 489c >T、C . 795a >G、C . 957a >C、C . 1023c >T、C . 1056a >G、C . 1353a >G。利用TaqI酶对SNP C . 957a >C进行PCR-RFLP验证，得到3个基因型，即AA型、AC型和CC型，等位基因频率A和C在巴厘牛中分别为0.10和0.90，而在PO和Limousine牛中为单态。SNP C . 957a >C PLAG1基因的基因型关联与出生体重(BW0)、断奶体重(WW205)、365日龄体重(YW365)、730日龄体重(YW730)和平均日增重(ADG)无相关性。SNP C . 957a >C作为巴厘牛生长性状的候选标记，有待进一步研究。

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来源期刊

Journal of the Indonesian Tropical Animal Agriculture AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers. The journal covers all aspect relating to Animal Agriculture, including: -Animal breeding and genetics -Animal feeding and nutrition -Animal reproduction -Animal biotechnology -Animal physiology -Animal production and technology -Animal products technology -Animal management and economics -Animal products processing and animal by-products -Animal microbiology -Livestock farming systems -Other related topics in relation to animal science. The topics of research are not only on Indonesian tropical animal agriculture, but also on other tropical regions of the world.