CAPSULAR SWELLING AND PASSIVE HAEMAGGLUTINATION INDUCED BY MONOCLONAL IgM REACTING WITH ACID POLYSACCHARIDES OF KLEBSIELLA

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 2009-08-15 DOI:10.1111/J.1699-0463.1975.TB01613.X

M. Harboe, J. Deverill, J. Eriksen

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引用次数: 2

Abstract

(1) Three selected macroglobulinaemia sera, Ro, Th, and We gave highly specific precipitin reactions in gel against type 12, type 13 and type 35 acid polysaccharides of Klebsiella. The same specificity was observed in more sensitive passive haemagglutination tests. The activity was confined to the monoclonal IgM present in the sera, and all of the monoclonal IgM in each serum had the characteristic activity. (2) The monoclonal IgM proteins induced typical capsular swelling reactions of similar specificity with Klebsiella bacteria. (3) In both precipitation, passive haemagglutination and capsular swelling reactions, the IgM Ro reacted both with type 12 and type 13 polysaccharides. Absorption experiments in situ in crossed immunoelectro-phoresis indicated that the same macroglobulin molecules reacted both with type 12 and type 13 polysaccharides. This cross-reaction evident in monoclonal IgM has not been seen previously in immune antibodies'against Klebsiella type 12 and 13.

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单克隆IgM与克雷伯菌酸性多糖反应诱导荚膜肿胀和被动血凝

(1)选取三种巨球蛋白血症血清，Ro、Th和We对克雷伯氏菌的12型、13型和35型酸性多糖进行了高度特异性的凝胶沉淀反应。在更敏感的被动血凝试验中观察到相同的特异性。活性仅限于血清中存在的单克隆IgM，每种血清中的单克隆IgM均具有特征活性。(2)单克隆IgM蛋白诱导克雷伯氏菌典型的囊膜肿胀反应，特异性相似。(3)在沉淀、被动血凝和荚膜肿胀反应中，IgM - Ro均与12型和13型多糖发生反应。交叉免疫电泳原位吸收实验表明，相同的巨球蛋白分子可与12型和13型多糖发生反应。这种交叉反应在单克隆IgM中很明显，以前在针对克雷伯氏菌12型和13型的免疫抗体中未见过。

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Acta pathologica et microbiologica Scandinavica. Section C, Immunology

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