Sulforaphane Attenuates AOM/DSS-Induced Colorectal Tumorigenesis in Mice via Inhibition of Intestinal Inflammation.

IF 2 4区医学 Q3 NUTRITION & DIETETICS

Nutrition and Cancer-An International Journal Pub Date : 2024-01-01 Epub Date: 2023-12-27 DOI:10.1080/01635581.2023.2274622

Fang Shao, Jie Pan, Yewen Xie, Jun Ding, Xiao Sun, Lei Xia, Dawei Zhu, Shizhong Wang, Chunjian Qi

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Abstract

Sulforaphane (SFN) is a compound derived from cruciferous plants. It has received considerable attention in recent years due to its effectiveness in cancer prevention and anti-inflammatory properties. The purpose of this study was to evaluate the antitumor potential of sulforaphane on colitis-associated carcinogenesis (CAC) through the establishment of a mouse model with AOM/DSS. First, AOM/DSS and DSS-induced model were established and administered SFN for 10 wk, and then the severity of colitis-associated colon cancer was examined macroscopically and histologically. Subsequently, immune cells and cytokines in the tumor microenvironment (TME) were quantified. Finally, the influence of sulforaphane was also investigated using different colon cell lines. We found that sulforaphane treatment decreased tumor volume, myeloid-derived suppressor cells (MDSC) expansion, the expression of the proinflammatory cytokine IL-1β, and the level of IL-10 in serum. Also, it enhanced the antitumor activities of CD8+ T cells and significantly reduced tumorigenesis as induced by AOM/DSS. SFN also attenuated intestinal inflammation in DSS-induced chronic colitis by reshaping the inflammatory microenvironment. This work demonstrates that sulforaphane suppresses carcinogenesis-associated intestinal inflammation and prevents AOM/DSS-induced intestinal tumorigenesis and progression.

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磺基拉芬通过抑制肠道炎症减轻AOM/DSS诱导的小鼠结肠肿瘤发生。

硫醚（SFN）是一种从十字花科植物中提取的化合物。近年来，由于其在癌症预防和抗炎方面的有效性，它受到了相当大的关注。本研究的目的是通过建立AOM/DSS小鼠模型来评估莱菔硫素对结肠炎相关致癌作用（CAC）的抗肿瘤潜力。首先，建立AOM/DSS和DSS-诱导的模型，并给予SFN 10周，然后从宏观和组织学角度检查结肠炎相关结肠癌癌症的严重程度。随后，对肿瘤微环境（TME）中的免疫细胞和细胞因子进行定量。最后，还使用不同的结肠细胞系研究了萝卜硫素的影响。我们发现，莱菔硫醚治疗降低了肿瘤体积、骨髓源性抑制细胞（MDSC）扩增、促炎细胞因子IL-1β的表达以及血清中IL-10的水平。此外，它增强了CD8+T细胞的抗肿瘤活性，并显著减少了AOM/DSS诱导的肿瘤发生。SFN还通过重塑炎症微环境来减轻DSS诱导的慢性结肠炎中的肠道炎症。这项工作表明，萝卜硫素抑制致癌相关的肠道炎症，并防止AOM/DSS诱导的肠道肿瘤发生和发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nutrition and Cancer-An International Journal 医学-营养学

CiteScore

5.80

自引率

3.40%

发文量

172

审稿时长

3 months

期刊介绍： This timely publication reports and reviews current findings on the effects of nutrition on the etiology, therapy, and prevention of cancer. Etiological issues include clinical and experimental research in nutrition, carcinogenesis, epidemiology, biochemistry, and molecular biology. Coverage of therapy focuses on research in clinical nutrition and oncology, dietetics, and bioengineering. Prevention approaches include public health recommendations, preventative medicine, behavior modification, education, functional foods, and agricultural and food production policies.