Effect of drought on aquaporin expression in grafted and ungrafted grapevine cultivars

IF 1.1 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY
M. Koç, R. Cangi, K. Yıldız
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引用次数: 1

Abstract

Drought stress severely affects growth, development and productivity in most agricultural crops. Since ancient times, rootstocks have been used to enable crop cultivation in unsuitable soil conditions. In the present study, three factors were evaluated: 1) cultivar: Vitis vinifera L. cv. ‘Horozkarası’ (drought-tolerant) and cv. ‘Kabarcık’ (drought-sensitive) were used; 2) rootstock: each cultivar was self-rooted and grafted onto ‘Rupestris du Lot’ rootstock; 3) drought stress: half of each cultivar/rootstock combination underwent drought stress and the other half was irrigated at field capacity for seven days. In order to estimate the responses of the cultivars, relative water content, proline content and aquaporin isoform expression levels (VvPIP2;1, VvPIP2;2, VvTIP1;1, and VvTIP2;1) were quantified. The results revealed that drought stress caused more reduction in relative water content (RWC) in ‘Kabarcık’ cultivar (drought-sensitive) than in ‘Horozkarası’ cultivar (drought-tolerant). Proline content increased in both cultivars in response to drought stress but to a relatively greater extent in the grafted ‘Kabarcık’ cultivar. Considering expression levels of genes, VvPIP2;1, VvPIP2;2, and VvTIP2;1 were downregulated whilst VvTIP1;1 was upregulated in the leaf. Both ‘Horozkarası’ and ‘Kabarcık’ cultivars showed similar trends in terms of their responses to drought stress. Grafting significantly increased the proline content in both cultivars exposed to drought stress. The rootstock conferred better drought protection to ‘Kabarcık’ cultivar than to ‘Horozkarası’ cultivar.
干旱对嫁接和未嫁接葡萄品种水通道蛋白表达的影响
干旱胁迫严重影响大多数农作物的生长、发育和生产力。自古以来,砧木就被用来在不适宜的土壤条件下种植作物。本研究主要评价三个因素:1)葡萄品种:葡萄(Vitis vinifera L. cv);' horozkarasir '(耐旱)和cv。使用' Kabarcık '(干旱敏感);2)砧木:各品种自根嫁接到‘Rupestris du Lot’砧木上;3)干旱胁迫:每个品种/砧木组合的一半经受干旱胁迫,另一半按田间容量灌溉7 d。为了评估不同品种的反应,我们定量测定了相对含水量、脯氨酸含量和水通道蛋白异构体表达水平(VvPIP2;1、VvPIP2;2、VvTIP1;1和VvTIP2;1)。结果表明,干旱胁迫导致“Kabarcık”(干旱敏感型)品种相对含水量(RWC)的降低幅度大于“horozkarasir”(耐旱型)品种。在干旱胁迫下,两个品种的脯氨酸含量均有所增加,但嫁接的Kabarcık品种的脯氨酸含量增加幅度较大。考虑基因的表达水平,在叶片中,VvPIP2;1、VvPIP2;2和VvTIP2;1下调,而VvTIP1;1上调。“horozkaras'”和“Kabarcık”品种在对干旱胁迫的响应方面表现出相似的趋势。嫁接显著提高了干旱胁迫下两个品种的脯氨酸含量。该砧木对' Kabarcık '品种的抗旱保护效果优于' horozkaras' '品种。
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来源期刊
Ciencia E Tecnica Vitivinicola
Ciencia E Tecnica Vitivinicola Agricultural and Biological Sciences-Food Science
自引率
12.50%
发文量
5
期刊介绍: Ciência e Técnica Vitivinícola (Journal of Viticulture and Enology) is an international journal that publishes original articles, research notes and review articles, written in Portuguese or in English, on the various fields of the science and technology of vine and wine: Viticulture, Enology and Vitivinicultural economy.
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