Detection and analysis of MMP-2 and MMP-9 in seminal plasma

IF 0.6 4区医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Journal of Mens Health Pub Date : 2012-12-01 DOI:10.1016/j.jomh.2012.07.002

Jiu-wei Du , Ke-yi Xu , Li-yi Fang , Xin-Lan Qi

{"title":"Detection and analysis of MMP-2 and MMP-9 in seminal plasma","authors":"Jiu-wei Du , Ke-yi Xu , Li-yi Fang , Xin-Lan Qi","doi":"10.1016/j.jomh.2012.07.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>The aim of this study was to explore the association between semen parameters and matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expression in human seminal plasma samples.</p></div><div><h3>Methods</h3><p><span>Conventional semen analysis was performed on semen samples (</span><em>n</em>  <span>64). MMP-2 and MMP-9 expression in seminal plasma was determined using gelatin zymography.</span></p></div><div><h3>Results</h3><p>Compared to semen samples with a normal sperm count, semen samples with a low sperm count (≤ 19×10<sup>6</sup>/ml) showed reduced sperm viability, a reduced percentage of Grade A sperm, a reduced percentage of morphologically-normal sperm, and lower proMMP-9 and MMP-9 but higher proMMP-2 and MMP-2 levels (<em>P</em>  <0.05).</p></div><div><h3>Conclusions</h3><p>MMP-2 and MMP-9 are both present in human semen, and low-concentration semen samples have increased MMP-2 and decreased MMP-9. We also observed relationships between MMP-2, MMP-9 and seminal parameters.</p></div>","PeriodicalId":54391,"journal":{"name":"Journal of Mens Health","volume":"9 4","pages":"Pages 216-219"},"PeriodicalIF":0.6000,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jomh.2012.07.002","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Mens Health","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1875686712000760","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}

引用次数: 3

Abstract

Background

The aim of this study was to explore the association between semen parameters and matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expression in human seminal plasma samples.

Methods

Conventional semen analysis was performed on semen samples (n = 64). MMP-2 and MMP-9 expression in seminal plasma was determined using gelatin zymography.

Results

Compared to semen samples with a normal sperm count, semen samples with a low sperm count (≤ 19 × 10⁶/ml) showed reduced sperm viability, a reduced percentage of Grade A sperm, a reduced percentage of morphologically-normal sperm, and lower proMMP-9 and MMP-9 but higher proMMP-2 and MMP-2 levels (P < 0.05). There were correlations between MMP-2 and MMP-9 expression and the percentage of Grade A sperm and morphologically-normal sperm (P < 0.05).

Conclusions

MMP-2 and MMP-9 are both present in human semen, and low-concentration semen samples have increased MMP-2 and decreased MMP-9. We also observed relationships between MMP-2, MMP-9 and seminal parameters.

查看原文本刊更多论文

精浆中MMP-2和MMP-9的检测与分析

本研究旨在探讨精液参数与人精浆中基质金属蛋白酶-2 (MMP-2)和基质金属蛋白酶-9 (MMP-9)表达的关系。方法对64份精液样本进行常规精液分析。明胶酶谱法测定精浆中MMP-2和MMP-9的表达。结果与正常精子数量相比，低精子数量(≤19 × 106/ml)的精子活力降低，a级精子比例降低，形态正常精子比例降低，proMMP-9和MMP-9水平降低，而proMMP-2和MMP-2水平升高(P <0.05)。MMP-2和MMP-9的表达与A级精子和形态正常精子的比例(P <0.05)。结论smmp -2和MMP-9均存在于人精液中，低浓度精液中MMP-2升高，MMP-9降低。我们还观察了MMP-2、MMP-9与种子参数之间的关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Mens Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-

CiteScore

0.90

自引率

28.60%

发文量

153

审稿时长

>12 weeks

期刊介绍： JOMH is an international, peer-reviewed, open access journal. JOMH publishes cutting-edge advances in a wide range of diseases and conditions, including diagnostic procedures, therapeutic management strategies, and innovative clinical research in gender-based biology. It also addresses sexual disparities in health, life expectancy, lifestyle and behaviors and so on. Scientists are encouraged to publish their experimental, theoretical, and descriptive studies and observations in as much detail as possible.