P132

Abstract

Purpose

To research the characteristics of the inflammation immune disorders and cell destruction at the local level in the lacrimal and intraocular liquids in patients with choroidal melanoma.

Materials and methods

Investigation of concentration of the cytokines interleukin (IL) IL-4, IL-6, IL-8, IL-10, autoantibodies to antigens of native DNA (AAB to Ag of nDNA) in the lacrimal and intraocular liquids was performed in 36 patients (72 eyes) aged 34–83 years. The mean age of the study group patients was 60.45 years (15 males and 21 females). As standard indicators, survey results of 20 “healthy” donor lacrimal liquid – volunteers were used. For statistical data processing, descriptive statistics and methods of inter-group comparisons were used. Quantitative characteristics are presented as the median (25, 75 percentile). For comparison of quantitative traits, regression analysis was used. P value of <0.05 considered significant (95% confidence interval). Statistical analysis was performed using the software R (Team RC, 2013).

Results

A significant positive relation of concentration of IL-4, IL-6, IL-8, IL-10, AAB to Ag of nDNA in lacrimal liquid of eye with choroidal melanoma and paired “healthy” eye was reveled (p < 0.05). Concentrations of IL-6, IL-8, IL-10 and AAB to Ag of nDNA in the lacrimal liquid of the eye with choroidal melanoma were significantly higher as compared with the levels of the control group (p < 0.05). The level concentration of IL-4 in lacrimal liquid in eye with choroidal melanoma was significantly low (p < 0.05). The level concentration of IL-6 was significantly higher in the early (T1–T2) than in the later stages (T3–T4) of choroidal melanoma development (p < 0.05). All stages of choroidal melanoma were characterized by higher level of IL-6, compared with the control group (p < 0.05). Given the role of IL-6 in the pathogenesis of malignant diseases, the activity may be indicative of tumor progression in the early stages of development of choroidal melanoma. A significant increase in IL-10 was shown at stages T1 and T4 compared with the control group (p < 0.05). Regression analysis revealed a significant positive relation of concentration of IL-4, IL-6, IL-8, IL-10, AAB to Ag of nDNA between lacrimal liquid and intraocular liquids of the eye with choroidal melanoma (p < 0.05).IL-4 r = 0,84 (0.58; 1.47), p < 0.05 IL-6 r = 0.98 (0.64; 1.32), p < 0.01 IL-8 r = 0.82 (0.12; 1.73), p = 0.05 IL-10 r = 0.84 (0.01; 1.81), p = 0.05AAB to Ag of nDNA r = 0.79 (0.36; 1.25), p < 0.05.

Conclusions

• 1.It was established that in the pathogenesis of choroidal melanoma, local Inflammation plays an important role as evidenced by a significant high concentration of IL-6, IL-8 due to lower levels of IL-4 and expressed immune disorders, cell destruction, as evidenced by significantly high level of AAB to Ag of nDNA, compared with the control group.

• 2.Use of lacrimal liquid is suitable for the study of the inflammation, immune disorders and cell destruction at the local level in patients with choroidal melanoma.

• 3.Significant increase in IL-6 level in lacrimal liquid was observed for all stages of choroidal melanoma, however, it was more pronounced in early stages (T1–T2), than in later stages (T3–T4).

• 4.A significant increase in IL-10 may indicate the severity of the local immunosuppression induced by tumor growth in the mechanisms of choroidal melanoma. A significant positive relationship between the concentrations of IL-4, IL-6, IL-8, IL-10, AAB to Ag of nDNA in the lacrimal liquids of eye and paired “healthy” eye was found in patients with choroidal melanoma.