Nicholas A. Stover, Andre R.O. Cavalcanti
{"title":"Using NCBI BLAST","authors":"Nicholas A. Stover, Andre R.O. Cavalcanti","doi":"10.1002/9780470089941.et1101s08","DOIUrl":null,"url":null,"abstract":"<p>BLAST is the most widely used software in bioinformatics research. Its main function is to compare a sequence of interest, the query sequence, to sequences in a large database. BLAST then reports the best matches, or “hits,” found in the database. This simple program has two primary applications. First, if the function of the query sequence is unknown, it may be possible to infer its function based on the recognized functions of similar sequences. Second, if the researcher has a query sequence with a known function, it may be possible to identify sequences in the database that have similar functions. The utility of BLAST therefore depends on the researcher's choice of query sequence and database. An appreciation for the functions and limitations of BLAST are vital to using this program effectively. This unit will introduce the basic concepts behind BLAST, walk through BLAST searching protocols, and interpret common results. <i>Curr. Protoc. Essential Lab. Tech</i>. 8:11.1.1-11.1.35. © 2014 by John Wiley & Sons, Inc.</p>","PeriodicalId":500994,"journal":{"name":"Current Protocols Essential Laboratory Techniques","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2014-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9780470089941.et1101s08","citationCount":"14","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols Essential Laboratory Techniques","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/9780470089941.et1101s08","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 14
Abstract
BLAST is the most widely used software in bioinformatics research. Its main function is to compare a sequence of interest, the query sequence, to sequences in a large database. BLAST then reports the best matches, or “hits,” found in the database. This simple program has two primary applications. First, if the function of the query sequence is unknown, it may be possible to infer its function based on the recognized functions of similar sequences. Second, if the researcher has a query sequence with a known function, it may be possible to identify sequences in the database that have similar functions. The utility of BLAST therefore depends on the researcher's choice of query sequence and database. An appreciation for the functions and limitations of BLAST are vital to using this program effectively. This unit will introduce the basic concepts behind BLAST, walk through BLAST searching protocols, and interpret common results. Curr. Protoc. Essential Lab. Tech. 8:11.1.1-11.1.35. © 2014 by John Wiley & Sons, Inc.
使用NCBI BLAST
BLAST是生物信息学研究中应用最广泛的软件。它的主要功能是将感兴趣的序列(查询序列)与大型数据库中的序列进行比较。然后BLAST报告在数据库中找到的最佳匹配或“命中”。这个简单的程序有两个主要的应用。首先，如果查询序列的功能未知，则可以根据类似序列的识别功能来推断其功能。其次，如果研究人员有一个具有已知功能的查询序列，则有可能在数据库中识别具有类似功能的序列。因此，BLAST的效用取决于研究者对查询序列和数据库的选择。了解BLAST的功能和局限性对于有效地使用该程序至关重要。本单元将介绍BLAST背后的基本概念，通过BLAST搜索协议，并解释常见的结果。咕咕叫。Protoc。基本的实验室。科技,8:11.1.1-11.1.35。©2014 by John Wiley &儿子,Inc。
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