Methods of Inducing Inflammatory Bowel Disease in Mice

Abstract

Animal models of experimentally induced inflammatory bowel disease (IBD) are useful for understanding more about the mechanistic basis of the disease, identifying new targets for therapeutic intervention, and testing novel therapeutics. This unit provides detailed protocols for five widely used mouse models of experimentally induced intestinal inflammation: chemical induction of colitis by dextran sodium sulfate (DSS), hapten-induced colitis via 2,4,6-trinitrobenzene sulfonic acid (TNBS), Helicobacter-induced colitis in mdr1a ^−/− mice, the CD4⁺ CD45RB^hi SCID transfer colitis model, and the IL-10^−/− colitis model. © 2016 by John Wiley & Sons, Inc.