Jan Vrána, Petr Cápal, Hana Šimková, Miroslava Karafiátová, Jana Čížková, Jaroslav Doležel
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引用次数: 24
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Abstract
Analysis and sorting of plant chromosomes (plant flow cytogenetics) is a special application of flow cytometry in plant genomics and its success depends critically on sample quality. This unit describes the methodology in a stepwise manner, starting with the induction of cell cycle synchrony and accumulation of dividing cells in mitotic metaphase, and continues with the preparation of suspensions of intact mitotic chromosomes, flow analysis and sorting of chromosomes, and finally processing of the sorted chromosomes. Each step of the protocol is described in detail as some procedures have not been used widely. Supporting histograms are presented as well as hints on dealing with plant material; the utility of sorted chromosomes for plant genomics is also discussed. © 2016 by John Wiley & Sons, Inc.
植物染色体的流动分析与分选
植物染色体的分析和分选(植物流式细胞遗传学)是流式细胞术在植物基因组学中的一个特殊应用，其成功与否关键取决于样品质量。本单元以逐步的方式描述方法，从诱导细胞周期同步性和有丝分裂中期分裂细胞的积累开始，继续制备完整的有丝分裂染色体悬液，染色体的流动分析和分选，最后处理分选的染色体。由于有些程序尚未广泛使用，因此对协议的每个步骤都进行了详细描述。提供了支持直方图以及处理植物材料的提示;还讨论了染色体分类在植物基因组学中的应用。©2016 by John Wiley &儿子,Inc。
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