A Simple Light Stimulation of Caenorhabditis elegans

Kun He Lee, Michael Aschner
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引用次数: 6

Abstract

Response via noxious stimulus can be an important indicator of sensory neuron function and overall health of an organism. If the stimulation is quick and simple, and the animal can be rescued afterwards, such a method not only allows for assays pertaining to changed sensory ability after various treatments, but also increases the reliability of the statistical relationships that are established. This protocol demonstrates a stimulation assay in Caenorhabditis elegans, using blue light from common laboratory equipment: the fluorescent microscope. The nematode detects blue light using a set of amphid ciliary sensory neurons, and blue light is detrimental to its overall health after a prolonged exposure. However, under brief exposure, blue light stimulation provides a rapid and easy method for quantifying sensory functions and health without harming the animal. © 2016 by John Wiley & Sons, Inc.

秀丽隐杆线虫的简单光刺激
对有害刺激的反应是生物体感觉神经元功能和整体健康状况的重要指标。如果刺激是快速和简单的,并且动物可以在之后获救,这种方法不仅允许对各种治疗后感觉能力的变化进行分析,而且还增加了所建立的统计关系的可靠性。本方案演示了秀丽隐杆线虫的刺激试验,使用来自常见实验室设备:荧光显微镜的蓝光。线虫使用一组两栖动物纤毛感觉神经元来检测蓝光,长时间暴露在蓝光下对其整体健康有害。然而,在短时间照射下,蓝光刺激提供了一种快速简便的方法来量化动物的感觉功能和健康状况,而不会伤害动物。©2016 by John Wiley &儿子,Inc。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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