Hydraulic role in differential stomatal behaviors at two contrasting elevations in three dominant tree species of a mixed coniferous and broad-leaved forest in low subtropical China

Abstract

Quantifying the variation in stomatal behavior and functional traits of trees with elevation can provide a better understanding of the adaptative strategies to a changing climate. In this study, six water- and carbon-related functional traits were examined for three dominant tree species, Schima superba, Pinus massoniana and Castanopsis chinensis, in a mixed coniferous and broad-leaved forest at two elevations (70 and 360 ​m above sea level, respectively) in low subtropical China. We hypothesized that trees at higher elevations would develop more efficient strategies of stomatal regulations and greater water transport capacity to cope with more variable hydrothermal conditions than those at lower elevations. Results show that the hydraulic conductivity did not differ between trees at the two elevations, contrary to our expectation. The C. chinensis trees had greater values of leaf mass per unit area (LMA), and the S. superba and C. chinensis trees had greater values of wood density (WD), relative stem water content (RWC), and ratio of sapwood area to leaf area (H_v) at the 360-m elevation than at 70-m elevation. The mean canopy stomatal conductance was greater and more sensitive to vapor deficit pressure at 360 ​m than at 70 ​m for both S. superba and C. chinensis, while stomatal sensitivity did not differ between the two contrasting elevations for P. massoniana. The midday leaf water potential (ψ_L) in P. massoniana was significantly more negative at 360 ​m than at 70 ​m, but did not vary with increasing elevation in both S. superba and C. chinensis. Variations in H_v can be related to the differential stomatal behaviors between the two elevations. The variations of stomatal behavior and ψ_L with elevation suggested the isohydric strategy for the two broad-leaved species and the anisohydric strategy for the conifer species. The species-specific differences in LMA, WD, RWC, and H_v between the two elevations may reflect conservative resource use strategies at the higher elevation. Our findings revealed a close relationship between hydraulic and stomatal behavior and may help better understand the functional responses of forests to changing environmental conditions.