CHARACTERIZATION OF Cochliobolus sativus AND Pyrenophora teres FUNGI BELONGING TO THE LEAF SPOT COMPLEX OF BARLEY (Hordeum vulgare) ISOLATED FROM BARLEY SEEDS IN MEXICO

IF 0.3 Q4 AGRONOMY

Chilean Journal of Agricultural & Animal Sciences Pub Date : 2021-03-01 DOI:10.29393/chjaas37-29cctj50029

T. Romero-Cortes, E. A. Zavala-González, V. H. Pérez España, J. E. Aparicio-Burgos, Jaime Alioscha Cuervo-Parra

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引用次数: 1

Abstract

Barley ( Hordeum vulgare ) is generally used for animal feed, and food and beer production industries. Its production is affected by phytopathogens, of which Ramularia collo-cygni, Puccinia spp., Pyrenophora teres and Cochliobolus sativus are of global concern. In Mexico, there are few studies available on the morphological and molecular identification of fungi responsible for causing fungal diseases of barley. Therefore, the objective of this work was to identify, morphologically and molecularly, fungi isolated from barley seeds. The strains were identified by morphological analysis and by sequencing of the conserved ITS1/5.8s/ITS2 region of the ribosomal gene. Morphologically, the strains were identified as P. teres and C. sativus , which was confirmed with bioinformatics techniques using BLAST and MEGA6 programs. The results showed that the strains isolated from barley seeds in the highlands of Mexico were consistent with the sequences of P. teres and C. sativus deposited in the GenBank. These results will allow identifying some of the species of native fungi found in barley seeds.

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从墨西哥大麦种子中分离出的大麦（Hordeum vulgare）叶斑复合体中的胭脂虫和圆叶拟除虫菊真菌的特征

大麦（大麦）通常用于动物饲料、食品和啤酒生产行业。它的生产受到植物病原体的影响，其中木霉属（Ramularia collo cygni）、Puccinia spp.、Pyrenophora teres和Cochliobolus sativus是全球关注的。在墨西哥，很少有关于引起大麦真菌病的真菌的形态学和分子鉴定的研究。因此，本工作的目的是从大麦种子中分离出真菌，从形态学和分子生物学角度进行鉴定。通过形态学分析和核糖体基因的保守ITS1/5.8s/ITS2区域的测序来鉴定菌株。在形态学上，菌株被鉴定为P.teres和C.sativus，这通过使用BLAST和MEGA6程序的生物信息学技术得到了证实。结果表明，从墨西哥高原大麦种子中分离到的菌株与GenBank中保存的P.teres和C.sativus序列一致。这些结果将有助于鉴定大麦种子中发现的一些本土真菌。

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来源期刊

Chilean Journal of Agricultural & Animal Sciences AGRONOMY-

CiteScore

0.70

自引率

0.00%

发文量

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