Effect of platelet-rich plasma on bone regenerate consolidation in distraction osteogenesis: An experimental study in rabbits.

IF 1.1 4区医学 Q3 ORTHOPEDICS

Acta orthopaedica et traumatologica turcica Pub Date : 2022-01-01 DOI:10.5152/j.aott.2022.20443

M. Karakayali, Yakup Alpay, B. Sarısözen

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引用次数: 2

Abstract

OBJECTIVE The aim of this study was to determine the effect of platelet-rich plasma (PRP) on bone regenerate consolidation in a rabbit model of distraction osteogenesis. METHODS In this study, 12 male New Zealand rabbits weighing 1600 to 2000 g were used. All the rabbits were randomly divided into two groups (n = 6 per group): PRP group and control group. A two-ring, circular external fixator was applied to the right tibia of each rabbit in both groups. After corticotomy, all the tibiae were distracted at a rate of 0.5 mm/day for 20 days. PRP was injected to the osteotomy sites on the 7th, 14th, and 21st days postoperatively. Mineral density of the new bone tissue formed in the distraction zone was measured using quantitative computed tomography in the 3rd, 4th, and 5th weeks. At the end of the 6th week, the animals were sacrificed, and the specimens were evaluated biomechanically and histologically. RESULTS Microcomputed tomography assessment showed significant bone mineral density increase from the 3rd to the 6th week (62.3% and 43.7% for the PRP and control groups, respectively). In the PRP group, the measurements on the 3rd, 4th, and 5th weeks were 416 ± 29, 487 ± 9.9, and 675 ± 37.8 HU (Hounsfield units), respectively, whereas in the control group were 313, 374, and 450 HU, respectively. In the comparison of weekly measurements of the two groups, the increase in bone density in the PRP group was higher than that in the control group (P < 0.001). During the mechanical tests, in the PRP group, the mean torsion was 46.50°and the mean torque 0.53 Nm, while in the control group, the mean torsion was 19.33°and the mean torque 0.65 Nm. The mechanical analysis of the groups revealed significant differences in the mean maximum torsion angles (P = 0.024). The histological examination showed that both groups had external and internal periosteal calli. Callus tissue in four rabbits in the PRP group and two rabbits in the control group was remodeled; normal bone formation occurred and distracted bone ends were completely healed. CONCLUSION The results of the present study have indicated that PRP injection can enhance bone regenerate consolidation and increase bone mineral density during the healing process of distraction osteogenesis.

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富血小板血浆对兔牵张成骨过程中骨再生固结的影响：一项实验研究。

本研究的目的是确定富血小板血浆（PRP）在兔牵张成骨模型中对骨再生固结的影响。方法本研究选用12只雄性新西兰兔，体重1600～2000g。将所有家兔随机分为两组（每组n=6）：PRP组和对照组。两组兔的右胫骨均采用双环圆形外固定器。皮质切开术后，所有胫骨以0.5毫米/天的速度分散20天。术后第7天、第14天和第21天在截骨部位注射PRP。在第3、4和5周，使用定量计算机断层扫描测量在牵张区形成的新骨组织的矿物密度。在第6周结束时，处死动物，并对标本进行生物力学和组织学评估。结果显微计算机断层扫描评估显示，从第3周到第6周，骨密度显著增加（PRP组和对照组分别为62.3%和43.7%）。PRP组第3、4和5周的测量值分别为416±29、487±9.9和675±37.8 HU（Hounsfield单位），而对照组分别为313、374和450 HU。在两组每周测量值的比较中，PRP组的骨密度增加高于对照组（P<0.001）。在机械测试中，PRP-组的平均扭转为46.50°，平均扭矩为0.53 Nm，而对照组的平均扭曲为19.33°，平均转矩为0.65 Nm。力学分析显示，两组的平均最大扭转角存在显著差异（P=0.024）。PRP组的4只兔子和对照组的2只兔子的愈伤组织被重塑；骨形成正常，分散的骨端完全愈合。结论本研究结果表明，在牵张成骨愈合过程中，注射PRP可增强骨再生固结，增加骨密度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta orthopaedica et traumatologica turcica ORTHOPEDICS-

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Acta Orthopaedica et Traumatologica Turcica (AOTT) is an international, scientific, open access periodical published in accordance with independent, unbiased, and double-blinded peer-review principles. The journal is the official publication of the Turkish Association of Orthopaedics and Traumatology, and Turkish Society of Orthopaedics and Traumatology. It is published bimonthly in January, March, May, July, September, and November. The publication language of the journal is English. The aim of the journal is to publish original studies of the highest scientific and clinical value in orthopedics, traumatology, and related disciplines. The scope of the journal includes but not limited to diagnostic, treatment, and prevention methods related to orthopedics and traumatology. Acta Orthopaedica et Traumatologica Turcica publishes clinical and basic research articles, case reports, personal clinical and technical notes, systematic reviews and meta-analyses and letters to the Editor. Proceedings of scientific meetings are also considered for publication. The target audience of the journal includes healthcare professionals, physicians, and researchers who are interested or working in orthopedics and traumatology field, and related disciplines.