Taxonomic diversity and succession of bacterial consortium from Kombucha

Abstract

The aim of the present work was to analyze the taxonomic diversity in the Kombucha bacterial consortium during long-term cultivation in the North (Arctic) of the European part of Russia. The high-performance sequencing showed that 99.1 % of the bacterial fraction of the fresh 7-d culture was Proteobacteria of mostly cellulose-producing genera with the order Acetobacterales being dominant and represented by the Komagataeibacter (87.3 %) and Gluconobacter (6.3 %). Aging of the Kombucha bacterial consortium from 20 to 90-d cultivation lead to the reduction of number of cellulose-producing bacteria and intense growth of cellulolytic bacteria including Clostridiales, Bacteroidetes, Actinomyces. Also the acidophilic microorganisms have been detected. The long-term growth of the Kombucha bacterial consortium can be considered as a succession of microbial communities.