Effects of Long Non-coding Ribonucleic Acid Plasmacytoma Variant Translocation 1 Regulating Octamer Binding Transcription Factor 4 Expression on Hepatocellular Carcinoma Cells

Abstract

The researchers aimed to evaluate the effects of long non-coding ribonucleic acid plasmacytoma variant translocation 1 (lncRNA PVT1) regulating octamer binding transcription factor 4 (OCT4) expression on hepatocellular carcinoma (HCC) cells. It was discovered that in contrast to the blank group, si-PVT1 group had lower PVT1 and OCT4 expressions, number of invading cells and wound healing rate, and higher radiosensitivity (P<0.05). OCT4 served as a gene targeted by lncRNA PVT1. The PVT1 group exhibited declined luciferase activity compared with the NC group after transfection with wild-type OCT4 (P<0.05). In comparison with those of the sh-NC group, the number of invading cells and wound healing rate dropped in the sh-OCT4 group (P<0.05). At the same radiation dose, a lower survival fraction than that in the sh-NC group was observed in the sh-OCT4 group (P<0.05). Silencing lncRNA PVT1 significantly suppresses HCC cell invasion as well as migration and elevates their radiosensitivity, probably by down-regulating OCT4 expression.